多功能蛋白聚糖Versican与TNF-α在胆囊癌组织的表达及意义  

Expressions of Versican and TNF-α in gallbladder carcinoma tissue and their significance

作　　者：李福利[1] 方征[2] 夏咸军[2] 刘洪[2] 赵海滨[2,3] 罗昆仑[1,2] 

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]中国人民解放军第一0一医院肝胆外科,江苏无锡214044 [3]中国人民解放军第一0一医院病理科,江苏无锡214044

出　　处：《中国普通外科杂志》2016年第2期225-230,共6页China Journal of General Surgery

基　　金：南京军区医学科技创新课题资助项目(14ZD05)

摘　　要：目的:探讨多功能蛋白聚糖Versican与TNF-α在胆囊癌组织中的表达及意义。方法:应用免疫组化方法检测并比较Versican与TNF-α在62例胆囊癌组织(肿瘤组)、30例胆囊结石伴慢性炎症的胆囊组织(炎症组)、20例正常胆囊组织(正常组)中的表达,分析两者与胆囊癌患者临床病理特征及预后的关系。结果:Versican与TNF-α在正常组、炎症组、肿瘤组中阳性表达率均呈依次逐渐增高,差异均有统计学意义(均P<0.05);Versican与TNF-α在胆囊癌组织中的表达呈正相关(r=0.501,P<0.05)。Versican与TNF-α的表达均与患者TNM分期、淋巴结转移有关,且Versican还与胆囊癌分化程度有关(均P<0.05)。生存分析显示,Versican与TNF-α阳性表达患者生存率均明显低于各自阴性患者(χ2=5.240、5.493,均P<0.05),但两者均阳性患者的生存率与两者单一阳性的患者差异无统计学意义(均P>0.05)。结论:Versican与TNF-α可能在胆囊癌的发生发展过程中起重要作用,且Versican与TNF-α的阳性表达为胆囊癌的不良预后因素。Objective： To investigate the expressions of Versican and TNF-α in gallbladder carcinoma and their signii cance. Methods： The expressions of Versican and TNF-α in specimens of 62 gallbladder carcinoma tissues（tumor group）, 30 gallbladder tissues with gallstones and chronic inflammation（inflammation group） and 20 normal gallbladder tissues（normal group） were detected by immunohistochemical staining and compared, and the relations of their expressions with clinicopathologic variables and prognosis of gallbladder carcinoma patients were analyzed.Results： Both positive expression rates of Versican and TNF-α presented an ascending order in normal group, inflammation group and tumor group, with statistical difference（all P〈0.05）; there was a positive correlation between the Versican and TNF-α expression in gallbladder carcinoma tissue（r=0.501, P〈0.05）. Either Versican or TNF-α expression was signii cantly related to lymph node metastasis and TNM stage of the patients, and the Versican expression was also related to the degree of dif erentiation of gallbladder carcinoma（all P〈0.05）. Survival analysis showed that the survival rate in patients with positive Versican or TNF-α expression was significantly lower than that in their negative counterparts（χ2=5.240, 5.493, both P〈0.05）, but the survival rate in patients with positive expressions of both Versican and TNF-α had no statistical dif erence compared with those with the positive expression of either of them alone（both P〉0.05）. Conclusion： Versican and TNF-α may probably play an important role in the process of occurrence and development of gallbladder carcinoma, and the positive expression of Versican and TNF-α may predict a poor prognosis.

关 键 词：胆囊肿瘤 多功能蛋白聚糖类 肿瘤坏死因子Α 预后 

分 类 号：R735.8[医药卫生—肿瘤]