紧密连接蛋白Claudin-5表达在乳腺癌组织中的表达及其临床意义  

Expression and clinical significance of claudin-5 in breast cancer

作　　者：李丹[1] 赵敬生[1] 

机构地区：[1]宝鸡市妇幼保健院乳腺外科,陕西宝鸡721000

出　　处：《临床肿瘤学杂志》2016年第2期142-145,共4页Chinese Clinical Oncology

摘　　要：目的探讨人乳腺癌组织中紧密连接蛋白Claudin-5的表达及与临床病理特征的关系。方法应用半定量PCR和Western blotting检测88例乳腺癌组织和61例乳腺增生组织中Claudin-5 m RNA及Claudin-5蛋白的表达水平,分析乳腺癌组织中Claudin-5蛋白表达与临床病理特征(分化程度、淋巴结转移、年龄、组织学分级、肿瘤大小和临床分期)及预后的关系。结果乳腺癌组织中的Claudin-5 m RNA和Claudin-5蛋白相对表达水平分别为0.87±0.13和0.95±0.23,均高于乳腺增生组织的0.23±0.09和0.54±0.18,差异有统计学意义(P<0.01);Claudin-5蛋白表达与分化程度有关(P<0.05),与年龄、肿瘤大小、临床分期和组织学分级无关(P>0.05);Claudin-5蛋白低表达者的中位生存期为45.5个月,高于高表达者的29.5个月,差异有统计学意义(P<0.05)。结论 Claudin-5在乳腺癌组织中高表达,可能与乳腺癌发生和转移有着密切关系,并可作为判断乳腺癌淋巴结转移和预后评估的参考指标。Objective To explore the expression and clinical significance of claudin-5 in breast cancer. Methods The ciau- din-5 mRNA and protein expression levels of 88 breast cancer tissues and 61 hyperplasia of mammary tissues were detected by semi- quantitative RT-PCR and Western blotting. The association between claudin-5 levels and elinieopathological characteristics（ differentia- tion degree, lymph node metastasis, age, histological grade, tumor size and clinical stage） was analyzed statistically as well as the prognosis. Results The mRNA and protein level of elaudin-5 in the tissues of breast cancer patients were （0. 87±0. 13 ） and （0. 95± 0. 23 ） , higher than （0.23 ±0. 09） and （0. 54±0. 18 ） of hyperplasia of mammary tissues with significant difference （P〈0. 01 ）. Claudin- 5 expression was related with differentiation grade （P〈0.05）, but without age, tumor size and clinical stages （P〉0. 05 ）. The median o- verall survival was 45.5 months for patients with low expression of claudin-5 protein, higher than 29.5 months for patients with high ex- pression of elaudin-5 （P〈0. 05）. Conclusion There was a relative high expression of elaudin-5 in breast cancer. Claudin-5 expression may be associated with breast cancer occurrence and progression, and may serve as a potential indicator for lymphatic metastasis and prognosis in breast cancer.

关 键 词：乳腺癌 乳腺增生 紧密连接蛋白Claudin-5 

分 类 号：R737.9[医药卫生—肿瘤]