NtC3H基因对烟草类黄酮及绿原酸合成的影响  

The Influence of Nt C3H on the Synthesis of Flavonoids and Chlorogenic Acid in Tobacco

作　　者：李洋[1] 唐雪冰 李晓峰 李明[1] 储昭辉[1] 丁新华[1] 

机构地区：[1]作物生物学国家重点实验室山东省农业微生物重点实验室山东农业大学,山东泰安271018 [2]黑龙江烟草工业有限责任公司海林卷烟厂,黑龙江海林157100

出　　处：《中国烟草科学》2016年第1期8-13,共6页Chinese Tobacco Science

基　　金：国家高技术研究发展计划"富含类黄酮及咖啡酰奎尼酸番茄品种的培育"(863;2010AA10Z103)

摘　　要：为验证Nt C3H是否参与烟草中类黄酮和绿原酸等次生代谢物质的合成,构建了p CXSN::Nt C3H超量表达载体,利用农杆菌介导的叶盘转化法将Nt C3H基因转入野生型烟草三生烟,并通过HPLC检测转基因烟草中绿原酸以及芦丁、山奈酚芸香苷等类黄酮的含量变化。结果显示,与野生型三生烟草相比,转基因烟草叶片中绿原酸及芦丁的含量最高,分别提高了3.6倍和6.1倍,山奈酚芸香苷含量提高了24.6倍。研究结果证实了Nt C3H基因参与烟草中类黄酮和绿原酸等次生代谢物质的合成。To test whether NtC3H participates in the biosynthesis of flavonoids, and chlorogenic acid (CGA) in tobacco and increases the contents of these secondary metabolites, we constructed the over-expression vector pCXSN::NtC3H and introduced it into Nicotiana tabacum var. samsun via Agrobacterium-mediated transformation. The concentrations of the different secondary metabolites including CGA, rutin, and kaempferol rutinoside in leaves of wild-type and transgenic lines were determined by HPLC. Compared to wild-type samsun tobacco, in transgenic tobacco the concentrations of CGA were increased 3.6-fold, rutin and kaempferol rutinoside were increased 6.1- and 24.6-fold respectively. These results suggested that NtC3H positively regulated the synthesis of flavonoids and CGA in tobacco.

关 键 词：烟草 NtC3H基因 绿原酸 类黄酮 

分 类 号：S572[农业科学—烟草工业]