5-脂氧合酶激活蛋白基因多态性与脑梗死易患性的临床研究  

Clinical research of the relationship between 5-1ipoxygenase activating protein gene polymorphism and cerebral infarction susceptibility

作　　者：冯学问 吴伟林 陈春友 仇晨峰 鲍贤俊 

机构地区：[1]温岭市第一人民医院神经内科,浙江省温岭317500

出　　处：《中国医师杂志》2016年第2期212-215,共4页Journal of Chinese Physician

基　　金：温岭市科技计划资助项目（2011WLCB0088）

摘　　要：目的探讨5-脂氧合酶激活蛋白基因（ALOXSAP）多态性在脑梗死患者中的表达与易患性的关系。方法分别选取脑梗死患者和体检健康人群各290例，应用聚合酶链反应-限制性片段长度多态性（PCR．RFLP）分析法，检测ALOXSAP基因的单倍体型HapA（SG13S114T、SG13S89G、SG13S32A、SG13S25G）、HapB（SG13S377A、SG13S114A、SG13S41A和SG13S35G）以及相关核苷酸位点的多态性。结果脑梗死患者与正常人群之间ALOXSAP的单核苷酸多态性（SNP）-SG13S114、SNP．SG13S32基因型及单倍体型HapA携带率比较差异均有统计学意义（P〈0．05）；其中SNP—SG13S1MAT与SNP—SG13S32AA均可独立增加脑梗死的患病风险（OR〉1．0，P〈0．05）。结论本地区脑梗死发病率受ALOXSAP的SNP—SG13S11g、SNP-SG13S32基因型及单倍体型HapA的影响。Objective To investigate the expression of 5-1ipoxygenase activating protein gene （ALOX5AP） polymorphism in the patients with cerebral infarction, and explore its relationship with cerebral infarction susceptibility. Methods Patients with cerebral infarction and healthy volunteers were selected for this study, whose venous blood was extracted and detected with polymerase chain reaction/restriction fragment length polymorphism （PCR-RFLP）. Haplotype A （SG13Sll4T, SG13S89G, SG13S32A, SG13S25G）, haplotype B （SG13S377A, SG13Sll4A, SG13S41A, SG13S35G）, and their nucleotide polymorphism loci were observed. Results Single nueleotide polymorphism （SNP）-SG13Sll4, SNP- SG13S32 and HapA carrying rate were significantly different between patients with cerebral infarction and healthy volunteers （ P 〈 0.05）. SNP-SG13Sll4 and SNP-SG13S32 were independent risk factors of cerebral infarction （ OR 〉 1.0, P 〈 0. 05）. Conclusions The morbidity of cerebral infarction in Wenling City was influenced by SNP-SG13S114, SNP-SG13S32, and HapA carrying rate.

关 键 词：花生四烯酸盐5-脂氧合酶/生物合成/遗传学 多态现象 遗传 脑梗死/遗传学 

分 类 号：R743.3[医药卫生—神经病学与精神病学]