小鼠皮肤损伤愈合过程中PI3K/Akt通路的作用  

Effects of PI3K/Akt Pathway in Wound Healing Process of Mice Skin

作　　者：高彦令 刘长胜[3] 赵锐[1] 王林林[1] 李姗姗[1] 刘敏[1] 张淼[1] 姜述堃 田志岭[1] 王檬[1] 官大威[1] 

机构地区：[1]中国医科大学法医学院法医病理学教研室,辽宁沈阳110001 [2]苏州市公安局刑警支队,江苏苏州215000 [3]鞍山市人民检察院技术处,辽宁鞍山114001

出　　处：《法医学杂志》2016年第1期7-12,共6页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81273342);鞍山市科学技术项目(2060499)

摘　　要：目的探讨小鼠皮肤损伤愈合过程中损伤区磷脂酰肌醇-3-激酶(phosphatidylinositol-3-kinase,PI3K)、磷酸化磷脂酰肌醇-3-激酶(phospho-PI3K,p-PI3K)、蛋白激酶B(protein kinase B,PKB,又称Akt)和磷酸化蛋白激酶B(phospho-Akt,p-Akt)的表达及其随时间的变化规律。方法应用免疫组织化学染色、Western印迹法和real-time PCR技术检测小鼠皮肤损伤不同时间段PI3K、Akt、p-PI3K及p-Akt的变化情况。结果免疫组织化学染色显示,皮肤损伤后,PI3K、p-Akt阳性染色出现在单核细胞和成纤维细胞,峰值出现在组织修复期。Western印迹法显示,各个时间段均有PI3K、p-PI3K、Akt、p-Akt的阳性条带,其中p-PI3K、p-Akt表达的峰值出现在炎症期和增生期,PI3K、Akt表达的高峰出现在组织重建期。real-time PCR结果显示,PI3K m RNA的峰值出现在炎症期和组织重建期,Akt m RNA的峰值出现在组织重建期。结论在皮肤损伤愈合过程中,PI3K、Akt、p-PI3K及p-Akt的表达变化呈现不同的规律性并有较好的时间依赖性,PI3K/Akt通路的表达规律可以用于法医学损伤时间推断。Objective To investigate the expressions and time-dependent changes of phosphatidylinositol-3- kinase （PI3K）, phospho-P13K （p-PI3K）, protein kinase B （PKB/Akt） and phospho-Akt （p-Akt） during wound healing process of mice skin. Methods The changes of PI3K, p-PI3K, Akt and p-Akt expression in skin wound were detected by immunohistochemistry, Western blotting and real-time PCR. Results Im- mtmohistochemistry showed the expression of PI3K and p-Akt were observed in mononuclear and fl- broblast after skin wound, and reached peak in reconstruction. The positive bands of PI3K, p-PI3K, Akt and p-Akt were observed in all time points of the wound healing process by Western blotting. The ex- pression peak of p-PI3K and p-Akt showed in inflammation and proliferation; the expression peak of PI3K and Akt in reconstruction. Real-time PCR showed the expression peak of PI3K mRNA in inflammation and reconstruction and the peak of Akt mRNA in reconstruction. Conclusion During the wound healing process, the expressions of PI3K, Akt, p-PI3K and p-Akt show different changes with significant corre- lation to wound time. The expression of PI3K/Akt may be a valuable marker for wound time estimation.

关 键 词：法医病理学 创伤和损伤 皮肤 磷脂酰肌醇-3-激酶 磷酸化磷脂酰肌醇-3-激酶 蛋白激酶B 磷酸化蛋白激酶B 小鼠 

分 类 号：R737.31[医药卫生—肿瘤]