响应面试验优化落叶松中二氢槲皮素纯化工艺  

Optimization of Purification Process for Dihydroquercetin in Larch Roots by Response Surface Methodology

作　　者：王佳奇[1] 宋明铭 陈凯[1] 王月亮 丁传波[1] 郑毅男[1] 申雷[1] 刘文丛[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品科学》2016年第4期20-24,共5页Food Science

基　　金：国家科技部科技型中小企业技术创新基金项目(13C26212201221)

摘　　要：目的:优选落叶松中二氢槲皮素的最佳纯化工艺。方法:在单因素试验的基础上,运用Box-Behnken响应面法设计料液比、结晶温度和结晶时间三因素三水平的试验模型,以二氢槲皮素的纯度作为评价指标,对二氢槲皮素的纯化工艺进行优化。结果:最佳纯化条件为料液比1∶12(g/mL)、结晶温度5℃、结晶时间20 h。结论:研究结果可为二氢槲皮素纯化工艺的确定以及工业生产提供科学依据。Objective： To optimize the purification conditions for dihydroquercetin in larch roots. Methods： Single factor method combined with Box-behnkn response surface methodology was used to optimize four factors including solid/liquid ratio,recrystallization temperature and time by establishing a model involvin g four independent variables at three levels each based on dihydroquercetin purity. Results： The optimal purification conditions were determined as recrystallized for 20h at 5 ℃ at a liquid-to-solid ratio of 1：12（dihydroquercetin：water）. Conclusion： The results of the study can provide scientific basis for the determination of the purification process of dihydroquercetin in industrial production.

关 键 词：二氢槲皮素 重结晶 响应面 

分 类 号：R931.6[医药卫生—生药学]