高效液相色谱法检测诺丽果浆及其中药复方保健品中莨菪亭  

Determination of Scopoletin in Noni Juice and Traditional Chinese Medicinal Compound Health Product Containing Noni Juice by HPLC

作　　者：陈晨[1] 刘平怀[1] 罗宁[1] 李昂[1] 何沂飞[1] 符晶[1] 

机构地区：[1]海南大学教育部热带多糖资源利用工程研究中心,热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《食品科学》2016年第4期83-87,共5页Food Science

基　　金：“十二五”国家科技支撑计划项目(2011BAD14B01);海南省中药现代化科技专项(ZY201327);国家科技型中小企业技术创新基金项目(13C26244604892);海南省产学研一体化项目(CXY20150034)

摘　　要：为研究诺丽果浆及其中药复方保健品中莨菪亭的高效液相检测方法,优化色谱条件:依利特Sino Chrom ODS-BP色谱柱(4.6 mm×200 mm,5μm),流动相为乙腈-0.1%磷酸溶液(30∶70,V/V),流速1 m L/min,柱温25℃,进样量20μL,检测波长348 nm。结果表明,莨菪亭在0.5~20μg/m L质量浓度范围内与其色谱峰峰面积线性关系良好,回归方程Y=73.86X-0.554(R^2=0.999),诺丽果浆和保健品中莨菪亭测定结果的平均回收率分别为104.05%和97.11%,相对标准偏差分别为0.81%和1.99%。该方法准确可靠,可用于定性、定量测定诺丽果浆及其保健品中的莨菪亭。A high performance liquid chromatography（HPLC） method was established for the determination of scopoletin in noni juice and a traditional Chinese medicinal compound health product containing noni juice. The chromatographic separation was performed on an Elite Sino Chrom ODS-BP column（4.6 mm×200 mm,5 μm） using acetonitrile-0.1%phosphoric acid aqueous solution（30：70,V/V） as the mobile phase at a flow rate of 1 mL/min. The column temperature was 25 ℃,and the detection wavelength was set at 348 nm. The results showed a good linear relationship between peak area and scopoletin concentration in the range of 0.5–20 μg/m L. The average recoveries for noni juice and the health product containing noni juice were 104.05% and 97.11%,with relative standard deviations（RSDs） of 0.81% and 1.99%,respectively. This method proved to be accurate and reliable and could be used to qualitatively and quantitatively determine scopoletin in noni juice and the compound health product containing noni juice.

关 键 词：诺丽果浆 保健品 莨菪亭 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]