蓝莓花青素提取物对H_2O_2诱导人胃癌BGC-823细胞氧化损伤的保护作用  

Protective effect of anthocyanins extract from blueberry on human gastric cancer BGC- 823 cell oxidative injury induced by H_2O_2

作　　者：张震[1] 周婷婷[2] 张华[2] 李亚晨[3] 

机构地区：[1]鞍山市肿瘤医院科教科,辽宁鞍山114034 [2]大连理工大学生命科学与技术学院,辽宁大连116024 [3]大连医科大学劳动卫生与环境卫生教研室,辽宁大连116044

出　　处：《大连医科大学学报》2016年第1期16-19,共4页Journal of Dalian Medical University

基　　金：辽宁省自然科学基金项目(2013023054)

摘　　要：目的在细胞水平上分析蓝莓花青素提取物的抗氧化活性。方法用6.25μg/m L、12.5μg/m L、25μg/m L、50μg/m L和100μg/m L蓝莓花青素提取物预处理人胃癌BGC-823细胞24 h后,再加入1 mmol/L H2O2诱导损伤2 h。按不同处理分为对照组、H2O2处理组和蓝莓花青素保护组。采用MTT比色法检测细胞活力、相差显微镜观察细胞形态、荧光探针DCFH-DA测定细胞活性氧(ROS)生成量、比色法和可见光法测定谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性,分析不同浓度的蓝莓花青素提取物预处理对H2O2诱导BGC-823细胞氧化损伤的影响。结果与H2O2处理组相比较,6.25μg/m L、12.5μg/m L、25μg/m L、50μg/m L和100μg/m L蓝莓花青素提取物预处理均能增加BGC-823细胞活力(P<0.05)。25μg/m L和100μg/m L蓝莓花青素提取物预处理能阻止H2O2诱导的BGC-823细胞形态损伤,抑制ROS生成,增加GSH-Px和CAT的活性。结论蓝莓花青素提取物对H2O2诱导人胃癌BGC-823细胞氧化损伤具有保护作用。Objective To explore the antioxidant activity of anthocyanins extract from blueberry on cells. Methods The human gastric cancer BGC- 823 cells were treated with anthocyanins extract from blueberry for 24 h with 6. 25 μg / m L,12. 5 μg / m L,25 μg / m L,50 μg / m L and 100 μg / m L,then treated with H2O2（ 1 mmol/L） for 2 h. The cell viability was measured by MTT assay,the cell morphological change observed by phase contrast microscope,reactive oxygen species（ ROS） measured with the fluorescent probe 2＇,7＇- dichlorofluorescin diacetate（ DCFH- DA）. The activity of glutathione peroxidase（ GSH- Px） and catalase（ CAT） were analyzed by the chromatometry and the visible spectrophotometry. Results Pretreatment with varied concentrations of anthocyanins extract from blueberry all increased BGC- 823 cell viability（ P 〈0. 05）. Pretreated with 25 μg/m L and 100 μg/m L anthocyanins extract inhibited the formation of ROS and elevated the activities of GSH- Px and CAT. Conclusion The anthocyanins extract from blueberry could protect human gastric cancer BGC- 823 cell to oxidative injury induced by H2O2.

关 键 词：蓝莓花青素提取物 BGC-823细胞 氧化损伤 抗氧化 

分 类 号：R151[医药卫生—营养与食品卫生学]