利用植物生长调节剂诱导烟草器官分化的基础教学实验  

Basic teaching experiment by using plant growth regulators in nicotiana tabacum

作　　者：李玉明[1] 王洪钟[1] 谢莉萍[1] 张贵友[1] 

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《实验技术与管理》2016年第1期32-34,39,共4页Experimental Technology and Management

摘　　要：在MS培养基中加入植物生长调节剂,诱导无菌烟草叶片的生长与分化,其中:2,4-D可以诱导叶片生成愈伤组织;NAA与6-BA浓度比为5∶1时,可以诱导叶片生成不定根;NAA与6-BA浓度比为1∶5时,可以诱导叶片生成不定芽。The growth and differentiation of sterile leaves could be induced by plant growth regulators. Callus was induced by 2, 4-D added in MS media. Adventitious roots were induced in MS media containing a mixture of NAA： 6-BA （5..1 concentration ratio）. While the adventitious buds were induced when the concentration ratio of NAA.. 6-BA was 1：5 in MS.

关 键 词：基础实验 组织培养 诱导分化 2 4-D NAA 6-BA 

分 类 号：Q943.1[生物学—植物学] G642.423[文化科学—高等教育学]