脑转移瘤动物模型的制备方法及研究进展  

Animal models of brain metastasis:preparation methods and research progress

作　　者：崔白苹 陈平 孙安阳[1] 

机构地区：[1]盐城卫生职业技术学院神经退行性疾病与修复实验室 [2]盐城市第一人民医院肿瘤科,江苏盐城224005

出　　处：《中国药理学通报》2016年第3期304-309,共6页Chinese Pharmacological Bulletin

摘　　要：脑转移瘤(brain metastasis)是指源于中枢神经系统以外的肿瘤细胞转移到脑组织的颅内常见恶性肿瘤。目前脑转移瘤造模有外周接种和直接脑内接种瘤细胞两大类方法。外周接种方法包括造模动物左心室或颈动脉内注射、原位接种等途径;脑内接种方法包括肿瘤细胞株直接接种于动物特定脑区的简化方法和临床脑转移瘤活检标本直接接种于裸大鼠脑内的新方法。该文综述了脑转移瘤模型的制备方法,包括可选用的接种动物、瘤细胞系、接种方法和模型的检测方法,并介绍这一领域的最新研究进展。Brain metastasis(BM) is a common brain tumor in adults,originating from extracranial location in the body.There has been a growing interest in producing suitable animal models for studying BM in vivo.Current BM models take peripheral or brain route for tumor cell inoculation,including intra-cardiac or intra-carotid artery injection or orthotopic injection.Direct implantation of patient tumor biopsies into rodent brain bears advantages of clinical relevance.This review presents a comprehensive introduction to key elements for establishing animal models of BM,with highlights on selections of suitable model animals,brain-seeking tumor cell lines, reasonable inoculation routes,as well as succedent phenotyping methods.In the end,perspectives and future directions in this field are discussed.

关 键 词：脑转移 肿瘤 动物模型 裸鼠 细胞接种 制备方法 检测方法 

分 类 号：R-05[医药卫生] R363-332