阿魏酸钠治疗糖皮质激素性骨质疏松的实验研究  

Effect of Sodium ferulate on glucocorticoid-induced osteoporosis

作　　者：刘洋[1] 陈珺[1] 翟玉莹[1] 叶小天 肖珊[1] 柯诗韵 李青南[1] 杨国柱[1] 卢丽[1] 陆幸妍[1] 

机构地区：[1]广东药科大学生命科学与生物制药学院/广东省生物技术候选药物研究重点实验室,广东广州510006

出　　处：《中国药理学通报》2016年第3期394-398,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金面上项目(No 30971172);广东省科技计划项目(No 2013B060300022)

摘　　要：目的探讨阿魏酸钠对于大鼠糖皮质激素性骨质疏松症的治疗作用。方法 3月龄Wistar大鼠随机均分5组:对照组、模型组、治疗组(阿魏酸钠低、中、高剂量组)。动物处死前进行钙黄绿素双荧光标记。采用骨组织形态计量学方法测量胫骨的静态参数、动态参数、骨组织细胞和生长板变化。结果①静态参数:与对照组相比,模型组骨小梁面积百分数、骨小梁厚度、骨小梁数量明显减少,骨小梁分离度增大;与模型组相比,阿魏酸钠中、高剂量组骨小梁面积百分数、骨小梁厚度、骨小梁数量明显增加,骨小梁分离度明显减小。低剂量组骨小梁厚度明显增加。②动态参数:与对照组相比,模型组标记周长百分数、骨转化率、骨表面新骨形成率明显增大;与模型组相比,中、高剂量组新骨年形成率增加。③骨组织细胞:与对照组相比,模型组单位骨小梁面积成骨细胞数量、破骨细胞数量以及成骨细胞周长占骨小梁周长百分率均明显增加。与模型组相比:各个实验组均没有明显变化。④生长板:与对照组相比,模型组生长板宽明显增大;与模型组相比,各实验组生长板退行细胞高度、生长板宽均没有明显变化。结论阿魏酸钠对糖皮质激素性骨质疏松的治疗作用主要体现在增加骨量和改善骨小梁结构,并促进骨形成。Aim To study the influence of Sodium ferulate( SF) on bone metabolism in glucocorticoid – induced osteoporosis rats.Methods Thirty cases of female Wistar Rats(3-month-old) were divided into control group, model group and SF group( low-dose group,middle-dose group,high-dose group) by randomized block design.Double fluorochrome labeling with calcein was performed before necropsy.The left tibia was taken for bone histomorphometry.Results In static parameters,the proximal tibia cancellous bone trabecular thickness,trabecular quantity and area ratio were significantly reduced in model group compared with control group; while compared with model group,those were increased in middle and high-dose SF group.Trabecular separation degree was increased in model group compared with control group,while it was decreased in middle and high-dose SF group compared with model group.In dynamic parameters,the calculation parameters of cancellous bone mark perimeter rate and the bone formation rate were increased in model group compared with control group, in middle and high-dose SF group the bone formation rate was increased compared with model group.In bone cells,osteoclast number per mm,osteoblast number per mm,percent osteoblast surface perimeter and percent osteoclast surface perimeter were increased in model group compared with control group.In growth-plate, the thickness of growth-plate was increased in model group compared with control group.In bone cells and growthplate there was no statistical significance between treatment group and model group.Conclusion This study demonstrates that SF can increase bone mass and improve bone structure,which may be related to the improvement of bone formation.SF is effective for GIOP in rats.

关 键 词：糖皮质激素性骨质疏松 阿魏酸钠 骨组织形态计量学 骨组织细胞 生长板 骨小梁 松质骨 

分 类 号：R-332[医药卫生] R323.72