血府逐瘀汤调控内皮祖细胞修复损伤血管内皮的研究  

Influence of Xuefuzhuyu decoction on EPC repairing injured vascular endothelium

作　　者：张伟[1] 刘晓丹[2] 李菲[2] 曹浪[2] 邓常清[2] 

机构地区：[1]湖南中医药大学附属岳阳医院,湖南岳阳414000 [2]湖南中医药大学医学院,湖南长沙410208

出　　处：《中国药理学通报》2016年第3期427-433,共7页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金青年项目(No 81000494);湖南省教育厅优秀青年基金(No 10B076)

摘　　要：目的在中医活血化瘀理论指导下,将中医治疗和细胞疗法相结合,探讨血府逐瘀汤调控内皮祖细胞(EPC)修复大鼠损伤血管内皮的作用,并阐释其机制。方法以内皮损伤大鼠为受试对象,灌胃血府逐瘀汤并静脉输注EPC,探讨该方联合EPC输注后,对大鼠内皮损伤模型血管内皮损伤的修复作用,并从细胞分子环节阐释其机制。结果与单用EPC组和单用药物组比较,血府逐瘀汤联合EPC组内膜厚度明显减少(P<0.05),降低甘油三酯、总胆固醇和升高高密度脂蛋白水平的作用更为明显(P<0.05),血钙下降更为明显(P<0.05),血管e NOS蛋白表达明显增加(P<0.05),血管SDF-1表达明显增高。结论血府逐瘀汤可促进EPC修复损伤的血管内皮,其作用机制可能与该方能改善机体内环境,促进EPC的归巢有关。Aim To investigate the role of Xuefuzhuyu decoction( XFZYD) combined with EPC in repairing damaged vascular endothelium using traditional Chinese medicine way of blood circulation combined with cell therapy.Methods The repaired situation of injuried endothelium was observed and the effect of XFZYD on EPC was analysed after the endothelial injuried rats were gavaged XFZYD and vena caudalis injected EPC.Results Compared with EPC group and XFZYD group,the XFZYD joint EPC group's endothelial thickness was reduced significantly(P<0.05).And there appeared more significant role in lowering triglycerides,total cholesterol and increasing HDL levels(P<0.05),the calcium was decreased more significantly(P<0.05); vascular e NOS protein expression increased significantly(P<0.05); vascular SDF-1 expression was significantly increased.Conclusion XFZYD can promote EPC repairing damaged endothelium,and the mechanism may be relevant to improving the environment and promoting the EPC homing.

关 键 词：血府逐瘀汤 内皮组细胞 血管内皮 ENOS SDF-1/CXCR-4 PI3K/磷酸化Akt 

分 类 号：R-332[医药卫生] R289.5