新疆阿魏乙酸乙酯部位质量控制方法研究  

Study on Quality Control of Ethyl-acetate Parts of Ferula Sinkiangensis

作　　者：张海英[1] 李伟[1] 周龙龙[2] 姜林[1] 周铭心[2] 

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830000 [2]新疆医科大学,新疆乌鲁木齐830011

出　　处：《中国中医药信息杂志》2016年第4期83-86,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81260625)

摘　　要：目的 建立新疆阿魏乙酸乙酯部位质量控制方法.方法 采用高效液相色谱法测定新疆阿魏乙酸乙酯部位的阿魏酸、法尼斯淝醇A、法尼斯淝醇C 含量.色谱柱为Waters XTerra RPC18（250 mm×4.6 mm,5 μm）,以乙腈-0.1%磷酸水为流动相,梯度洗脱,流速1.0 mL/min,检测波长324 nm,柱温30 ℃,进样量10 μL.结果 阿魏酸、法尼斯淝醇A、法尼斯淝醇C 分别在0.05～1.0、0.132～2.64、0.118～2.36 mg/mL 范围内线性关系良好,平均回收率分别为99.34%、98.96%、99.24%.新疆阿魏乙酸乙酯部位的阿魏酸、法尼斯淝醇A、法尼斯淝醇C 含量分别为33.4、76.5、72.3 mg/g.结论 该方法操作简单,结果准确,具有较好的重复性和稳定性,可为新疆阿魏乙酸乙酯部位的质量控制提供参考.Objective To establish the method for quality control of ethyl-acetate parts of Ferula sinkiangesis. Methods HPLC was used to detect the contents of ferulic acid, farnesiferol A and farnesiferol C. Waters XTerra RPC18 column (250 mm×4.6 mm, 5 μm) was used; acetonitrile-0.1% phosphoric acid was as mobile phase with gradient elution; flow rate was 1.0 mL/min; detective wavelength was 324 nm; temperature was 30℃; sample volume was 10 μL ResultsFerulic acid, farnesiferol A, and farnesiferol C showed a good linear relationship range from 0.05– 1.0 mg/mL, 0.132–2.64 mg/mL, and 0.118–2.36 mg/mL, respectively. The average recovery rates were 99.34%, 98.96% and 99.24% respectively. The contents of ferulic acid, farnesiferol A and farnesiferol C were 33.4, 76.5, 72.3 mg/g respectively.Conclusion The method was simple, accurate and repeatable, with good repeatability and stability, which can be used for the quality control of ethyl-acetate parts ofFerula sinkiangesis.

关 键 词：新疆阿魏 乙酸乙酯部位 阿魏酸 法尼斯淝醇A 法尼斯淝醇C 质量控制 

分 类 号：R284.1[医药卫生—中药学]