2-脱氧-D-葡萄糖联合顺铂对人宫颈癌Hela细胞增殖与凋亡的影响  

The effects of 2-deoxy-D-glucose and cisplatin combination on proliferation and apoptosis of the Hela cells

作　　者：朱国方[1] 王若雨[1] 李红霞[2] 孙英明[1] 秦建中[2] 刘庆平[3] 

机构地区：[1]大连大学附属中山医院肿瘤科,辽宁大连116011 [2]大连大学生命科学与技术学院,辽宁大连116600 [3]大连大学糖脂代谢研究重点实验室,辽宁大连116600

出　　处：《现代肿瘤医学》2016年第8期1187-1190,共4页Journal of Modern Oncology

基　　金：大连市科技计划项目(编号:2014E14SF147)

摘　　要：目的:研究2-脱氧-D-葡萄糖(2-deoxy-D-glucose,2-DG)与顺铂(Cisplatin,Cis-DDP)单独及联合对人宫颈癌Hela细胞增殖与凋亡的影响。方法:培养Hela细胞,2-DG、Cis-DDP单独及联合作用于Hela细胞,MTT法检测细胞的存活率;流式细胞仪检测各组药物处理后Hela细胞的凋亡率;ATP实验检测各处理组ATP生成情况;Western blot测定AKT、Caspase-3、PARP-1、MCL-1蛋白表达水平。结果:2-DG、Cis-DDP单独及联合作用Hela细胞均可降低细胞活性,呈时间剂量依赖性,联合用药组与其他各组存活率之间具有显著差异(P<0.05);用药组比对照组细胞ATP生成量降低但24h差异无统计学意义,48h联合用药组ATP生成量明显低于其他各组,且差异具有统计学意义(P<0.05);用药组细胞凋亡率均大于对照组,联合用药组凋亡率大于单药组,且具有明显差异(P<0.05);Western blot显示,联合用药可明显降低AKT、PARP-1、MCL-1蛋白的表达量,并使PARP-1、Caspase-3蛋白分解。结论:2-DG、Cis-DDP可有效抑制肿瘤细胞增殖,诱导其凋亡,2-DG联合Cis-DDP抗肿瘤作用明显增强。Objective： To study the effects of 2-deoxy-D-glucose（ 2-DG） single agent and combine with cisplatin on proliferation and apoptosis of cervical cancer cell line Hela. Methods： The cell viability of different drug disposal on Hela cells was analyzed by MTT assay. Flow cytometry was used to detect the apoptosis rate. ATP assay was used to detect the ATP synthesis. Western blot was applied to analyze the AKT,Caspase-3,PARP-1,MCL-1protein expression levels. Results： Both single or double-drug disposal can reduce the viability of the cells in a time-dependent manner,and the combination group presented a significantly different（ P〈0. 05） comparing with other groups. Drug disposal could reduce the synthesis of ATP,but no difference than control group in 24 h,however,the ATP synthesis for the combination group was obviously lower than any other group in 48 h which reached a statistically significant（ P〈0. 05）. For the apoptosis rate,drug disposal group was higher than the control group and combination group also showed effective advantage than single-agent group,both differences were statistically significant（ P〈0. 05）. WB result showed that the combination group could decrease the protein expressions of AKT,PARP-1,MCL-1,and cleave the PARP-1,Caspase-3. Conclusion： 2-DG and cisplatin can effectively inhibit tumor cell proliferation,induce apoptosis,and 2-DG combined with cisplatin provides obvious cisplatin anti-tumor effect promotion.

关 键 词：2-脱氧-D-葡萄糖 顺铂 HELA细胞 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]