miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响  

Effects of miR-760 on cell proliferation,migration and invasion of gastric cancer cell lines MGC-803

作　　者：李峰[1,2] 王芳[2] 梁爱华[1] 

机构地区：[1]山西大学生物技术研究所化学生物学与分子工程教育部重点实验室,山西太原030006 [2]中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2016年第2期207-210,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(31372199)

摘　　要：目的探讨miR-760对胃癌细胞系MGC-803增殖、迁移和侵袭的影响。方法 Real-time PCR分析50例胃癌组织(C)及其癌旁(N)中miR-760的表达水平;用pc DNA3.1载体构建过表达miR-760的重组质粒(pc DNA-miR-760),实现miR-760在MGC-803细胞中的过表达;分别用CCK-8法、Transwell和划痕实验检测细胞增殖、侵袭和迁移能力。结果与癌旁对照组相比,36例(72%)胃癌组织中出现miR-760的表达下调;过表达miR-760能显著抑制MGC-803细胞的迁移和侵袭能力(P<0.05),但对其增殖影响不大。结论 miR-760的表达下调可能与胃癌的进展有关,过表达miR-760可以抑制胃癌MGC-803细胞的迁移和侵袭。Objective To investigate the effects of miR-760 on cell proliferation,migration and invasion of gastric cancer cell line MGC-803. Methods The level of miR-760 expression was detected by real-time RT-PCR in 50 paired gastric cancer tissues and their adjacent normal tissues. miR-760 overexpression was achieved by transfection of construct pc DNA-miR-760 into MGC-803 cells. The proliferation,migration and invasion of MGC-803 cells were detected by the CCK-8,Transwell and scratch wound assay respectively. Results miR-760 decreased in 36cases（ 72%） of gastric cancer tissues compared to their control. Furthermore,overexpression of miR-760 effectively inhibited the migration and invasion of MGC-803 cells（ P 〈 0. 05）,but had no effects on proliferation.Conclusions Down-regulation of miR-760 has a correlation with the progression of gastric cancer. Up-regulation of miR-760 can inhibit the invasion and migration of MGC-803 cells.

关 键 词：miR-760 胃癌 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]