ADAM15、MMP-2和MMP-9在不同转移能力乳腺癌细胞系中的表达差异  

The expression of ADAM15,MMP-2 and MMP-9 in different breast cancer cell lines

作　　者：欧小波[1] 周密[2] 

机构地区：[1]遵义医学院珠海校区病理学教研室,广东珠海519041 [2]遵义医学院珠海校区临床医学系,广东珠海519041

出　　处：《基础医学与临床》2016年第2期227-231,共5页Basic and Clinical Medicine

基　　金：贵州省科学技术基金(黔科合J字LKZ【2011】40号)

摘　　要：目的分析ADAM15、MMP-2和MMP-9在不同转移能力乳腺癌细胞系中的表达,探讨其与乳腺癌细胞转移的相关性。方法 Transwell小室实验检测两种乳腺癌细胞系的转移能力,免疫细胞化学法对ADAM15、MMP-2及MMP-9在两种细胞中的表达进行定性、定位及半定量检测,Western blot对其进行定量检测。结果 MDA-MB-231乳腺癌细胞系的运动和侵袭能力均强于MCF-7乳腺癌细胞系。ADAM15、MMP-2及MMP-9在MDA-MB-231细胞系中的表达分别高于MCF-7细胞系(免疫细胞化学:P<0.001,P<0.05,P<0.001;Western blot:P<0.01,P<0.05,P<0.01),且三者在两系乳腺癌细胞表达的差异中,ADAM15表达的差异性最大,其次为MMP-9,MMP-2的差异性最小。结论 ADAM15、MMP-2及MMP-9均促进乳腺癌转移,且ADAM15与转移相关性最强而可能成为判断乳腺癌恶性生物学行为的指标。Objective Explore the expression of ADAM15,MMP-2 and MMP-9 in different breast cancer cell lines through experiment to investigate the relativity of the metastasis of breast cancer cell. Methods Transwell chamber experiments were used to test the metastasis of two breast cancer cell lines. Immunocytochemical were used to carry out qualitative analysis,localization and semi-quantitative detection and quantitative detection through Western blot for ADAM15,MMP-2 and MMP-9. Results The migration of MDA-MB-231 breast cancer cell line is stronger than the MCF-7. The expression of ADAM15,MMP-2 and MMP-9 in the MDA-MB-231 breast cancer cell line are higher than that in the MCF-7,and the difference of ADAM15 is more obvious than MMP-9 and MMP-2 in two cell lines. Conclusions The expression of ADAM15,MMP-2 and MMP-9 closely correlates to the metastasis of breast cancer cells,and infer that ADAM15 may have a closer relationship to the metastasis of breast cancer cells than the others.

关 键 词：ADAM15 MMP-2 MMP-9 乳腺癌细胞 转移 

分 类 号：R737.9[医药卫生—肿瘤]