桂皮酸抑制舌鳞癌细胞Tca8113的增殖  

Cinnamic acid inhibits the proliferation of Tca8113 cell

作　　者：凌佳璞 王雨坤 马义丽[1] 石昌荣 左晓娜[1] 黄健[1] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004 [2]广西大学生命科学与技术学院,广西南宁530000

出　　处：《基础医学与临床》2016年第2期237-242,共6页Basic and Clinical Medicine

基　　金：桂林市科学研究与技术开发计划(20130120-17);广西自然科学基金(2014GXNSFAA118236)

摘　　要：目的探讨桂皮酸(CINN)对舌鳞癌Tca8113细胞增殖的影响及机制。方法舌鳞癌细胞Tca8113经CINN处理后,倒置显微镜下观察细胞增殖和形态;MTT法检测细胞增殖;流式细胞术检测细胞周期;免疫组化检测KLF6、Bcl-2、P53、cyclin D1、c-Jun及PTEN的蛋白表达。结果 CINN呈时间-剂量依赖性显著抑制Tca8113细胞增殖(P<0.05);可见典型的Tca8113细胞分化的形态学特征;CINN处理后,G1、G2期细胞显著减少,S期细胞显著增加,细胞明显被阻滞于S期(P<0.05);CINN处理后KLF6和P53的蛋白表达上调(P<0.05),Bcl-2、cyclin D1及c-Jun的蛋白表达下调(P<0.05)。结论 CINN可抑制舌鳞癌细胞Tca8113的增殖并将其阻止于S期,同时诱导细胞凋亡。Objective To investigate the effect of cinnamic acid on the proliferation of tongue squamous cell carcinoma cell line Tca8113. Methods After treatment with cinnamie acid,the morphological changes of Tca8113 were observed. The proliferation inhibition of Tca8113 cell was detected by MTT method. The cell cycle of Tca8113 was analyzed by flow cytometry. The protein level of KLF6,Bcl-2,P53,cyclin D1,c-Jun and PTEN were measured by the method of immunohistochemistry. Results The proliferation of Tca8113 cells can be significantly inhibited by cinnnamic acid in vitro（ P 〈 0. 05）,and the inhibition is time and dose dependent; Typical feature of induced differentiation of Tca8113 cells was observed. After treatment with cinnamic acid,the cell number in G1 and G2phase decreased significantly while the number in S phase increased. Tca8113 cells significantly arrested in S phase（ P 〈 0. 05）. The protein level of KLF6 and P53 increased（ P 〈 0. 05） whereas the protein levels of Bcl-2,cyclin D1 and c-Jun decreased significantly（ P 〈 0. 05）. Conclusions Cinnamic acid inhibits the proliferation of Tca8113 cell by arresting the cell cycle in S phase,and then may induce Tca8113 cell to apoptosis finally.

关 键 词：舌癌细胞Tca8113 增殖抑制 桂皮酸 KLF6 

分 类 号：R739.8[医药卫生—肿瘤]