Research on Rapid Propagation and Domestication of Wild Raspberry Plantlets  

野生覆盆子种苗快繁与驯化试验研究（英文）

作　　者：朱伟俊[1] 徐森富[1] 陈依桃 

机构地区：[1]台州科技职业学院,浙江台州318020

出　　处：《Agricultural Science & Technology》2016年第2期298-300,共3页农业科学与技术（英文版）

基　　金：Supported by Taizhou College Students’Innovative Team Funding Project(classⅠ)~~

摘　　要：[Objective] This study was conducted to acquire a large quantity of wide raspberry plantlets and provide a basis for large-scale production. [Method] Re- search was performed on series tissue culture propagation of wide raspberry. [Re- sult] Adventitious shoots were induced with stem segments, and the best induction medium was MS ＋1.5 mg/L BA ＋0.3 mg/L NAA with an induction rate of 96%; and the best differentiation medium was MS ＋2.0 mg/L KT ＋0.5 mg/L NAA, which ex- hibited high root number and quality, with an average root number of 4.5 per plant. The substratum for training plantlets was leaf mould 60%＋perlite 30%＋vermiculite 10%; and the temperature of 23-27℃ and relative air humidity of 80%-90% were beneficial to survival of trained plantlets.[Conclusion] This study laid a foundation for further large-area cultivation of Rubus chingii Hu.[目的]为短期内获得大量优质覆盆子种苗及大规模生产提供依据。[方法]对野生覆盆子进行系列组培快繁研究。[结果]利用茎段能诱导出不定芽,最佳诱导培养基为MS＋1.5mg/L BA＋0.3 mg/L NAA,诱导率可达96%;最佳分化培养基为MS＋2.0 mg/L KT＋0.5 mg/L NAA,芽生长健壮,增殖达到4.2倍;最佳生根培养基为l/2 MS＋0.2 mg/L NAA,生根数量质量好,平均每株生根4.5条。炼苗基质以60%腐叶土＋30%珍珠岩＋10%蛭石为佳;炼苗温度23~27℃,空气相对湿度80%~90%有利于提高炼苗成活率。[结论]该试验为进一步进行掌叶覆盆子的大面积栽培奠定了基础。

关 键 词：Wild raspberry Tissue culture PROLIFERATION DOMESTICATION 

分 类 号：S567.19[农业科学—中草药栽培]