胃肠安及四藤方对人结肠癌细胞株干细胞CD133^+的影响  

Effect of Wei Chang An and Si Teng Fang on stem cells CD133^+ of human colon cancer strains

作　　者：祝利民[1] 沈克平[1] 周浩[1] 潘传芳[1] 姚琼[1] 

机构地区：[1]上海中医药大学附属龙华医院肿瘤科,上海200032

出　　处：《上海交通大学学报（医学版）》2016年第2期161-165,171,共6页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金青年基金项目(815034300)~~

摘　　要：目的观察胃肠安和四藤方对结肠癌细胞株HCT-116、SW480、HT-29及其干细胞CD133^+的抑制作用。方法人结肠癌细胞株(HCT-116、SW480、HT-29)随机分为对照组和不同浓度胃肠安(500 mg/L和1 000mg/L)、四藤方(100 mg/L和200 mg/L)干预组,干预24 h后,CCK-8法测定细胞活力,倒置显微镜观察细胞形态;流式细胞仪分析胃肠安和四藤方对HCT-116、SW480、HT-29中CD133^+表达的影响。结果 CCK-8法检测结果显示,胃肠安及四藤方干预HCT-116、SW480、HT-29细胞后,细胞活力均下降且呈剂量依赖性;倒置显微镜观察发现:胃肠安及四藤方干预24 h,HCT-116、SW480、HT-29的细胞数量减少,细胞体积缩小,遮光性差,细胞悬浮、脱落而死亡。流式细胞术分析结果显示:对照组HCT-116、SW480、HT-29中CD133^+细胞的比例分别为12.52%、10.98%、2.33%;胃肠安方1 000 mg/L干预24 h后,CD133^+细胞比例分别下降为7.49%、5.36%、0.11%;四藤方组200 mg/L干预24 h后,CD133^+细胞比例分别下降为7.83%、6.45%、0.18%。结论不同结肠癌细胞株中CD133^+细胞比例有差异。胃肠安及四藤方均具有抑制结肠癌细胞株HCT-116、SW480、HT-29增殖的作用;但在同样的干预时间内,较高浓度胃肠安(1 000 mg/L)及四藤方(200 mg/L)才具有抑制3种细胞株干细胞CD133^+表达的作用。Objective To observe the inhibitory effect of Wei Chang An and Si Teng Fang on colon cancer cell strains HCT-116,SW 480,and HT-29 and colon cancer stem cells CD133^＋.Methods Human colon cancer cell strains HCT-116,SW480,and HT-29 were randomly divided into the control group,WCA group（500mg/L and 1 000 mg/L）,and STF group（100 mg/L and 200 mg/L）.After 24 h of intervention,cell viability was detected by C C K-8 assay and cell morphology was observed by the inverted microscope.The effects of WCA and STF on the expression of CD133^＋ in HCT-116,SW480,and HT-29 were analyzed by flow cytometry.Results The results of CCK-8 assay showed that after HCT416,SW 480,and HT-29 cells were intervened by WCA and STF,their viability decreased in a dose-dependent manner.The observation results of the inverted microscope indicated that after 24 h of intervention by WCA and STF,the number of SW480,HT-29,and HCT-116 cells decreased,the cell volume reduced,light shading weakened,and cells suspended,detached,and died.The results of flow cytometry analysis showed that for the control group,proportions of CD133^＋ cells in cell strains HCT-116,SW480,and HT-29 were 12.52%,10.98%,and 2.33%,respectively.After 24 h of intervention by WCA of 1 000 mg/L,proportions of CD133^＋ cells dropped to 7.49%,5.36%,and0.11%.After 24 h of intervention by STF of 200 mg/L,proportions of CD133^＋ cells dropped to 7.83%and 6.45%,and 0.18%.Conclusion Proportions of CD133^＋ cells in different colon cancer cell strains were different.Both W C A and STF can inhibit the proliferation of colon cancer cell strains HCT-116,HT-29,and SW 480.But within the same intervention time,only higher concentration of WCA（1 000 mg/L） and STF（200 mg/L） can inhibit the expression of stem cells CD133^＋ of the three cell strains.

关 键 词：胃肠安 四藤方 结肠癌干细胞 CD133^+ 

分 类 号：R735.35[医药卫生—肿瘤]