拉米夫定对HepG2.2.15细胞MMP-9及p53表达的影响  

Effect of lamivudine on the expression of MMP-9 and p53 in Hep G2.2.15 cells

作　　者：王雪[1] 杨伟[2] 李靖[2] 马存花[1] 

机构地区：[1]潍坊医学院,山东潍坊261053 [2]解放军89医院,山东潍坊261021

出　　处：《实用医药杂志》2016年第2期151-153,共3页Practical Journal of Medicine & Pharmacy

摘　　要：目的通过检测拉米夫定作用于人肝癌细胞系(Hep G2.2.15)后对其基质金属蛋白酶-9(MMP-9)及p53表达的变化,探讨拉米夫定对原发性肝癌发生发展的抑制作用。方法不同浓度(100μg/ml、200μg/ml、300μg/ml)拉米夫定作用于Hep G2.2.15细胞不同时间(3 d及6 d)后,四甲基偶氮唑盐(MTT)比色法测定细胞增殖活性;双抗体夹心酶联免疫吸附实验(ELISA)测定细胞培养上清及胞质中MMP-9及p53蛋白含量。结果经拉米夫定作用后的Hep G2.2.15细胞生长未受明显抑制;细胞中MMP-9及p53的表达均出现不同程度降低。结论拉米夫定通过降低Hep G2.2.15细胞中MMP-9及p53的表达来抑制肝癌的侵袭及转移。Objective To study the inhibitory effect of lamivudine on the formation and development of primary liver cancer by detecting the expression of matrix metalloproteinases-9 （MMP-9） and p53 in human hepatocellular carcinoma cell line （HepG2.2.15）. Methods HepG2.2.15 were treated with different concentrations （100 μ/ml,200 μg/ml,300 μg/ml） of lamivudine for 3d and 6d in vitro,the cell proliferation activity of HepG2.2.15 were assayed bv MTT.Double antibody sandwich enzyme linked immunosorbent assay （ELISA） for the determination of MMP-9 and p53 in the cell culture supernatant and cytoplasm. Results The growth of HepG2.2.15 ceils was not significantly inhibited by lamivudine. The expression of MMP-9 and p53 appeared to decrease in different degrees. Conclusion Lamivudine inhibits the invasion and metastasis of HCC by reducing the expression of p53 and MMP-9 in HepG2.2.15 cells.

关 键 词：肝细胞肝癌 HEPG2.2.15细胞 MMP-9 P53 拉米夫定 

分 类 号：R735.7[医药卫生—肿瘤]