PDSW联合热疗对SGC-7901细胞PTEN和Ki67基因及蛋白表达的影响  

Effects of PDSW Combined with Hyperthermia Treats on PTEN, Ki67 Genes and Protein Expression of Gastric Cancer Cells SGC-7901

作　　者：唐辉蓉[1] 崔进[1] 张家骅[1] 代佑果[2] 李为明[1] 廖陈[1] 

机构地区：[1]昆明医科大学第二附属医院,昆明650101 [2]昆明医科大学第三附属医院,昆明650101

出　　处：《基因组学与应用生物学》2016年第1期21-27,共7页Genomics and Applied Biology

基　　金：云南省科学技术厅联合专项(2010CD166)项目资助

摘　　要：为探讨生理性深层海水(PDSW)联合热疗对人胃癌SGC-7901细胞体外和体内的抗癌作用,通过在体外培养人胃癌SGC-7901细胞株,随机分为实验组、对照组和空白组。实验组加PDSW,对照组加生理盐水,空白组不做其他处理。分别在37℃、40℃和43℃热疗温度下培养。同时,将SGC-7901细胞注射至裸鼠皮下,建立移植瘤模型,随机分为5组,至肿瘤长至直径为1 cm时,分别进行H_2O+RT、H_2O+40℃、H_2O+43℃、PDSW+40℃、PDSW+43℃处理,用实时荧光定量PCR检测细胞和组织中PTEN和Ki67基因表达情况,Western Blot检测PTEN和Ki67蛋白的表达情况。结果发现,PDSW联合热疗处理后,SGC-7901细胞和裸鼠皮下移植瘤组织中PTEN基因和蛋白的表达量上升幅度较大,而Ki67基因和蛋白的表达量较低,说明PDSW联合热疗能激活胃癌SGC-7901细胞中PTEN基因和蛋白上调表达,而抑制Ki67基因和蛋白的表达,从而有效抑制胃癌细胞的增殖。In order to explore the anti-cancer effects of PDSW combined with hyperthermia treats on cell viability and apoptosis of gastric cancer cells SGC-7901 in vitro and in vivo, the SGC-7901 cells were cultured in vitro, and then were randomly divided into experimental, control and blank groups. The experimental group received PDSW, and the control group was given normal saline, while the blank group had no other treatment. Each group were cultured respectively under 37℃, 40℃ and 43℃. At the same time, the SGC-7901 cells were injected into the nude mice to establish the model of transplantation tumor, and divided into 5 groups randomly to treatment in H20＋RT, H20＋40℃ ,H20＋43℃ ,PDSW＋40℃ ,PDSW＋43℃ when the tumour cells grew as long as 1 cm, by using RT-PCR to detect the gene expression and Western Blot to detect protein expression of Ki67 and PTEN. These results indicated that the expressions of PTEN gene and protein rised higher while the expressions of Ki67 gene and protein were lower in the SGC-7901 cell and transplantation tumor under the treatment of PDSW combined with hyperthermia. The above results showed that, the PDSW combined with hyperthermia can activate the PTEN gene and protein expression, and inhibit the expression of Ki67, which can effectively inhibit the proliferation of gastric cancer cells.

关 键 词：PDSW 热疗 SGC-7901 PTEN Ki67 qRT-PCR Western BLOT 

分 类 号：R735.2[医药卫生—肿瘤]