果蔗拔地拉及其芽变株系的SCoT、BPS和URP联合分子鉴定  

Molecular Identification of Fruit Sugarcane Badila and Its Bud Sports with Combination of SCoT, BPS and URP Markers

作　　者：刘俊仙[1,2] 熊发前[3] 刘欣[1,2] 刘丽敏[1,2] 罗丽[4] 刘红坚[1,2] 余坤兴[1,2] 淡明[1,2] 卢曼曼[1,2] 何毅波 李松[1,2] 

机构地区：[1]广西农业科学院甘蔗研究所,南宁530007 [2]广西蔗糖产业协同创制中心,南宁530007 [3]广西农业科学院经济作物研究所,南宁530007 [4]广西农业科学院农业科技信息所,南宁530007

出　　处：《基因组学与应用生物学》2016年第1期190-197,共8页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31501362;31401415);广西自然科学基金项目(2014GXNSFBA118289;2014GXNSFAA118090;2014GXNSFBA118068;2015GXNSFAA139063)共同资助

摘　　要：前期我们获得了果蔗拔地拉的4份自然芽变株系,本研究旨在揭示果蔗拔地拉及其自然芽变株系在DNA水平上的分子差异。综合采用基于单引物扩增反应的三种分子标记技术(SCoT,BPS和URP)的20条单引物对果蔗拔地拉及其自然芽变株系进行分子鉴定。结果表明:(1)在随机使用的20条单引物(3条SCoT标记单引物,5条BPS标记单引物和12条URP标记单引物)中,BPS9、URP6R和URP17R这三条单引物均扩增失败,未能扩增出任何条带,剩下的17条单引物总共扩增产生了89条条带,17条单引物中平均每条单引物扩增出5.24条条带;(2)3条SCoT标记单引物和5条BPS标记单引物均未能扩增产生出明显的可重复差异条带,只有URP标记单引物URP38F能扩增产生一条由亮度强弱及有/无同时存在引起的可重复差异条带,该条带大小约625 bp,该条单引物及其所产生的约625 bp大小的条带可用于果蔗拔地拉及其自然芽变株系的分子鉴定,能有效区分果蔗拔地拉及其自然芽变株系在DNA分子水平上显示出来的微小的遗传差异。Previously we had acquired four natural bud sports from fruit sugarcane badila. This study aimed to reveal the molecular differences between the fruit sugarcane badila and its four natural bud sports at the DNA level. Twenty single primers derived from three types of single primer amplification reaction based molecular marker techniques （SCOT, BPS and URP） were used to identify the molecular differences between the fruit sugarc- ane badila and its four natural bud sports at the DNA molecular level. The results showed that： 1） among the random used twenty single primers （three SCoT primers, five BPS primers, and twelve URP primers）, three single primers （BPS9, URP6R and URP17R） failed to amplify any bands and the remaining seventeen single primers gen- erated a total of 89 bands, with an average of 5.24 bands per primer; 2） all three single primers from SCoT marker technique and all five single primers from BPS marker technique failed to amplify obviously and reproducibly different bands, only one single primer called URP38F amplified one repeated different band which showed the different intensity of the brightness and presence and/or absence between the fruit sugarcane badila and its fournatural bud sports. The band sized approximately 625 bp. The single primer URP38F and the band sized approximately 625 bp could be used for molecular identification of fruit sugarcane badila and its four natural bud sports and effectively distinguish the tiny genetic differences between fruit sugarcane badila and its four natural bud sports at the DNA molecular level.

关 键 词：甘蔗 果蔗拔地拉 芽变株系 分子鉴定 SCoT BPS URP 分子标记 

分 类 号：S566.1[农业科学—作物学]