红外线照射对萎缩性胃炎大鼠胃黏膜表皮生长因子及其受体表达的影响  

The effect of infrared radiation on the expression of epidermal growth factor and its receptor in atrophic gastritis

作　　者：邵雪辉[1] 王建国[4] 韩志宏[5] 胡少龙[5] 张晓丽[2] 高秀芳[5] 杨跃平[3] 

机构地区：[1]河北北方学院医学物理教研室,张家口075000 [2]河北北方学院电镜室,张家口075000 [3]河北北方学院信息系,张家口075000 [4]张家口学院 [5]河北北方学院第三附属医院

出　　处：《中华物理医学与康复杂志》2016年第2期92-95,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：河北省科技支撑项目（14277778D）

摘　　要：目的研究红外线照射对实验性慢性萎缩性胃炎模型大鼠胃黏膜细胞表皮生长因子（EGF）及其受体EGFR表达的影响，并探讨其作用机制。方法采用随机数字表法将清洁级健康成年雄性Wistar大鼠40只分成正常对照组、模型对照组及红外线组，采用多因素综合法将模型对照组及红外线组大鼠制成慢性萎缩性胃炎动物模型，制模方法包括将30％酒精和2％水杨酸钠制成混合溶液，连续灌胃8周，每日1次，每次2ml，同时结合劳累（跑步）、饥饱失常（禁食）等多因素干预。待制模完成后，红外线组大鼠则给予红外线照射，每日照射1次，每次10min。于红外线连续照射20d后将各组大鼠处死，采用免疫组织化学法检测各组大鼠胃黏膜EGF及EGFR表达情况。结果正常对照组胃黏膜中EGF、EGFR表达[其阳性染色评分分别为（2．10±0．65）分、（4．20±2．24）分]较弱，模型对照组EGF、EGFR表达[其阳性染色评分分别为（4．91±2．58）分、（6．75±2．63）分]较正常对照组明显增强（P〈0．05）；红外线组EGF、EGFR表达[其阳性染色评分分别为（2．75±1．01）分、（4．75±1．66）分]较模型对照组明显减弱（P〈0．05），与正常对照组间差异无统计学意义（P〉0．05）。结论红外线照射能抑制慢性萎缩性胃炎大鼠胃黏膜中EGF、EGFR过度表达，在胃黏膜病变愈合过程中能发挥保护胃黏膜、促进组织修复等作用。Objective To study the effect of infrared irradiation on the expression of epidermal growth factor EGF） and epidermal growth factor receptor （EGFR） in the gastric mueosa of rats with chronic atrophic gastritis CAG） , and to explore its mechanism. Methods Forty healthy adult and clean Wistar male rats were randomized into a normal control group （N group） , a model control group （M group） and an infrared group （I group） using a random number table. CAG was induced in the rats of the M and I groups by intra-gastric infusion of 2 ml of a mixture of 30% alcohol and 2% sodium salicylate once every day for 8 weeks. This was combined with compulsory running and irregular fasting as pathogenic factors. After the CAG model had been established, the rats in the I group were given infrared irradiation for 10 min once every day for 20 days. Then all of the rats were sacrificed and the expression of EGF and EGFR was observed using immunohistochemistry. Results The positive staining scores for EGF and EGFR of the M group were 4.91±2.58 and 6.75±2.63, significantly higher than those of the N group （2.10±0.65 and 4.20±2.24 respectively）. Those of the I group were 2.75± 1.01 and 4.75± 1.66, significantly lower than those of the M group, though not significantly different from the N group＇s averages. Conclusion Infrared irradiation can inhibit over-expression of EGF and EGFR in the gastric mucosa of rats with chronic atrophic gastritis. This should protect the gastric mucosa and promote tissue repair and healing.

关 键 词：红外线 慢性萎缩性胃炎 表皮生长因子 表皮生长因子受体 大鼠 

分 类 号：R573.32[医药卫生—消化系统]