“素心”蜡梅花粉生活力及贮藏力的研究  

Studies on the Pollen Vitality and Storage Capacity in Chimonanthus praecox

作　　者：杨姗[1] 张家瑞[1] 秦垚[2] 马婧[1] 李名扬[1] 李志能[1] 

机构地区：[1]西南大学园艺园林学院重庆市花卉工程技术研究中心南方山地园艺学教育部重点实验室,重庆400715 [2]澳门科技大学商学院,澳门519020

出　　处：《重庆师范大学学报（自然科学版）》2016年第2期134-137,共4页Journal of Chongqing Normal University:Natural Science

基　　金：国家级大学生创新创业训练计划(No.201410635065);重庆市教委中小学创新人才培养工程(No.CY150210);西南大学第七届本科生科技创新基金(No.1319002);西南大学人才引进项目(No.SWU113030);中央高校基本业务费(No.XDJK2014B025;No.2362014xk10)

摘　　要：采用离体萌发法,研究不同的培养基对成年株"素心"蜡梅(Chimonanthus praecox)花粉萌发的作用,并探讨不同干燥时间及贮藏条件对花粉生活力的影响。结果表明,干燥4h的花粉在10%蔗糖+75mg·L^(-1) H_3BO_3+0.1‰CaCl_2+50mg·L^(-1) GA3的培养基上培养4h后萌发率最高,并且干燥4h后的花粉贮藏2d后生活力下降明显,在-20℃条件下贮藏6d后花粉萌发率明显高于4℃和20℃贮藏的花粉,第8d后3种贮藏条件下的花粉萌发率均不到1%。研究结果可为测定和分析蜡梅花粉生活力提供一定的参考,也为蜡梅储存指出了方向。By the means of germination in vitro, the effects of different culture media, preservation condition and drying time on the germination of pollens in Chimon anthus praecox‘Luteus＇ were studied. The results showed that the pollen dried for 4 hours and then incubated 4 hours on the medium, which is composed of 10% sucrose＋75 mg· L-1 H3BO3 ＋0. 1% CaCl2 ＋50 mg · L-1 GA3, reached the highest germination rate. Storing for two days, the vitality of pollen dried for 4 hours shows extinct descent. The germination rate of pollen stored at -20 ℃for 6 days was higher than that of at 4 ℃ and 20 ℃, respectively. The vitality of the pollen stored for 8 days is less than 1%. The results can provide a reference for the measurement and analysis of pollen viability,the orientation of pollen preservation in future is also suggested.

关 键 词：蜡梅 花粉 生活力 贮藏力 

分 类 号：S685.99[农业科学—观赏园艺]