辣椒素对非小细胞肺癌侵袭能力影响及其机制探讨  

Effects and Mechanism of Capsaicin on Invasion of Non-small Cell Lung Cancer

作　　者：任公平[1] 刘宏扬 于雪莲[1] 梁国英[3] 

机构地区：[1]牡丹江医学院红旗医院,黑龙江牡丹江157011 [2]齐齐哈尔市中医医院,黑龙江齐齐哈尔161000 [3]黑龙江中医药大学附属第一医院,哈尔滨150040

出　　处：《中国实验方剂学杂志》2016年第5期172-177,共6页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:观察辣椒素对人大细胞肺癌细胞NCI-H460侵袭能力及E钙黏蛋白(E-cadherin)蛋白,Slug蛋白表达的影响,探讨其抗非小细胞肺癌的可能机制。方法:体外培养NCI-H460细胞,以不同终浓度辣椒素进行处理,未加辣椒素为空白组。采用MTT比色分析法,观察辣椒素对NCI-H460细胞增殖抑制作用,计算半数抑制浓度(IC50);采用微丝荧光染色和Transwell小室细胞侵袭实验观察NCI-H460细胞侵袭能力,以及Western blot和RT-PCR分析检测E-cadherin和Slug蛋白及mRNA表达的变化。结果:MTT比色分析法结果显示,辣椒素对NCI-H460细胞生长具有明显抑制作用,与空白组比较,差异具有统计学意义(P<0.05);微丝荧光染色分析表明,辣椒素可明显抑制NCI-H460细胞丝状伪足形成;Transwell体外侵袭实验结果显示,辣椒素可显著抑制侵袭穿膜细胞数,与空白组比较,差异具有统计学意义(P<0.05)。Western blot和RT-PCR分析结果显示,辣椒素显著升高E-cadherin表达水平,并显著降低Slug表达水平,与空白组比较,差异具有统计学意义(P<0.05)。结论:辣椒素能明显抑制NCI-H460细胞活率,降低侵袭能力与其降低Slug表达,增加E-cadherin表达有关,可能是其抗非小细胞肺癌的机制之一。Objective： To investigate the effects of capsaicin on the invasion ability of human large cell carcinoma NCI-H460 and the expressions of E-cadherin and Slug, in order to discuss the possible mechanisms of anti-non-small cell lung cancer. Method： NCI-H460 cells were cultured in vitro and treated with capsaicin with various concentrations, with no capsacin in the blank group. The 50% inhibitory concentration（IC50） was measured by MTT method. The NCI-H460 cell invasion ability was evaluated by actin-tracker green fluorescent probe and transwell chamber invasion assay. Western blot and RT-PCR were used to detect the protein and mRNA expression of E-cadherin and Slug. Result： MTT assay results showed that the growth of NCI-H460 cells treated with capsaicin was significantly inhibited compared with the blank group, with statistically significant difference （P〈0.05）;Actin-tracker Green fluorescent probe showed that capsaicin can notably prohibit NCI-H460 filopodia formation. Transwell invasion in vitro results showed that capsaicin can significantly inhibit invasion of penetrating cells, with statistically significant difference compared with the control group （P〈0.05）. Western blot and RT-PCR analysis showed that E-cadherin expression level was significantly elevated and Slug expression level was significantly decreased by capsaicin, with statistically significant difference compared with the control group （P〈0.05）. Conclusion： Capsaicin can inhibit the NCI-H460 cell viability and invasion, its mechanism for anti-non-small cell lung cancer may be correlated with decrease in Slug expression and increase in E-cadherin expression.

关 键 词：辣椒素 侵袭能力 E钙黏蛋白 SLUG 

分 类 号：R285.5[医药卫生—中药学]