人乳头状瘤病毒L1壳蛋白表达与宫颈病变程度相关性研究  

Relationship research between expression of HPV L1 capsid protein and cervical lesions degree

作　　者：高家良 陈文虎[2] 孙爱华[2] 

机构地区：[1]宁波市鄞州人民医院,315040 [2]浙江医学高等专科学校,杭州310053

出　　处：《中华实验和临床病毒学杂志》2016年第1期34-36,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：浙江省卫生高层次创新人才培养工程项目

摘　　要：目的探讨人乳头瘤病毒（humanpa pillomavirus，HPV）感染宫颈上皮细胞L1壳蛋白表达对宫颈病变的进展及宫颈癌的发生的临床意义。方法病例组共259例，根据液基薄层细胞检测（TCT）结果不同分为未见上皮内病变细胞或恶性细胞（NILM）103例、非典型鳞状细胞（ASCUS）91例、低度鳞状上皮内病变（LSIL）36例、高度鳞状上皮内病变（HSIL）23例和鳞状细胞癌（SCC）6例。同时选择50例健康体检者作为对照组。分别用PCR-反向点杂交法检测HPV23种亚型和免疫细胞化学法检测L1壳蛋白表达。结果对照组PCR和L1壳蛋白表达结果均呈阴性。病例组NILM、ASC、LSIL、HSIL和SCC患者HPV感染率分别为23．3％、69．2％、83．3％、87．0％和100％，HPV—PCR阳性上述患者高危型HPV（HR—HPV）的构成比分别为79．2％、92．1％、96．7％、100％和100％。NILM、ASC、LSIL、HSIL和SCC患者HPVL1壳蛋白表达阳性率分别为25．2％、31．9％、63．8％、17．4％和0％。结论随宫颈病变的进展HPV感染率和HR．HPV构成比升高，提示本地区HPV感染率和HR—HPV构成比与宫颈上皮内瘤变程度密切相关。L1壳蛋白检测阳性率随病变早期表达呈上升趋势，晚期呈明显下降，HPVL1壳蛋白的表达与宫颈病变程度有关，可以作为宫颈癌癌前病变的随访指标。Objective To investigate the clinical significance of the expression of L1 capsid protein in cervical lesion cells infected with human papillomavirus （ HPV）, for the prevention of cervical cancer and suggestion of progression and probability of potential lesions. Methods 259 different levels of cervical lesion patients were patients group, including 103 cases negative for intraepithelial lesion and malignancy （NILM）, 91 cases with atypical squamous cells of undetermined significance（ASCUS） ,36 cases with lowgrade squamous intraepithelial lesion （LSIL）, 23 cases with high-grade squamous intraepithelial lesion （HSIL）, and 6 cases with squamous cell carcinoma （SCC）. Cervical cell samples of 50 normal physical examination people were control group. Immunohistochemical staining for L1 capsid protein and PCR-based reverse blot hybridization for 23 HPV DNA were employed. Results Both L1 capsid protein detection and PCR -based reverse blot hybridization detection were negative in the control group. The infection ratio of HPV were 23.3% , 69. 2% , 83.3%, 87. 0% and 100% in woman with NILM, ASCUS, LSIL, HSIL and SCC, respectively. The constituent ratio of high-risk HPV （HR-HPV） were 79. 2%, 92. 1%, 96. 7%, 100% and 100% in HPV positive patients with NILM, ASCUS, LSIL, HSIL and SCC, respectively. The positive rates of HPV LI capsid protein in NILM, ASCUS, LSIL, HSIL and SCC were 25.2% , 31.9% , 63.8% , 17.4% and 0.0% , respectively. Conclusions Both infection ratio of HPV and constituent ratio of HR-HPV were increased gradually with the aggravation of cervical lesions, and the infection ratio of HPV and constituent ratio of HR-HPV were closely related to the degree of cervical diseases. The detection results of L1 capsid protein were on the rise in the early phase of cervical lesion, however, declining in the late phase of cervical lesion, the expression of HPV L1 capsid protein associates with the degrees of cervical lesion, which may serve as an indicator of cervical precancerous lesions of the

关 键 词：乳头状瘤病毒 人 L1壳蛋白 免疫细胞化学 宫颈肿瘤 

分 类 号：R737.33[医药卫生—肿瘤]