miRNA qPCR检测方法研究进展及其应用  

Research Advance on miRNA qPCR Methods and Its Application

作　　者：冯世鹏[1] 

机构地区：[1]海南大学农学院海南省热带生物资源可持续利用重点实验室,海口570228

出　　处：《生物技术通报》2016年第2期59-69,共11页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31460178);海南省自然科学基金项目(314042);海南省教育厅项目(HNKY2014-16);海南大学科研启动项目(kyqd1306)

摘　　要：miRNA是目前国际研究的热点领域之一,而miRNA表达检测是miRNA研究的基础内容。针对miRNA序列短小的特点,开发出了多种检测方法,其中miRNA qPCR定量检测是应用最广泛的方法。就miRNA抽提,miRNA qPCR定量检测原理及miRNA qPCR定量检测流程包括引物设计、反应体系设置、内参基因筛选等方面进行了深入探讨,并对miRNA定量检测研究方法的发展趋势进行了展望,以期为研究者进行miRNA qPCR定量检测提供参考。miRNA is one of the hottest research fields in the world. The detection of mi RNA expression is the fundamental content in mi RNA research. Due to the feature of mi RNAs being short and small, varied methods were developed, and quantitative mi RNA q PCR is the most widely used one. This paper reviews the developments of mi RNA extraction method, quantitative detection principle of mi RNA q PCR, primer design, the reaction condition, and reference gene selection. The developments of mi RNA q PCR methods in the future are also discussed. All of these may provide a reference for researchers in the field when they detect mi RNA by q PCR method.

关 键 词：MIRNA STEM-LOOP RT-PCR poly(A)加尾法RT-PCR 延伸法RT-PCR 数字PCR 

分 类 号：Q503[生物学—生物化学]