云南高校图书馆联盟文献共享服务平台- STEM-LOOP

STEM-LOOP: 作品数：13被引量：34H指数：4; 导出分析报告; 相关领域：农业科学医药卫生更多>>; 相关作者：周莲莲韩东王修启朱晓彤束刚更多>>; 相关机构：四川农业大学中山大学华南农业大学吉林大学更多>>; 相关期刊：《Frontiers of Medicine》《生物技术通报》《Science China(Life Sciences)》《Acta Pharmacologica Sinica》更多>>; 相关基金：国家自然科学基金国家重点基础研究发展计划广东省自然科学基金海南省教育厅项目更多>>

Substitutions of stem-loop subdomains in internal ribosome entry site of Senecavirus A:Impacts on rescue of sequence-modifying viruses被引量：1: 《Journal of Integrative Agriculture》2024年第7期2391-2406,共16页Qianqian Wang Jie Wang Lei Zhang Xiaoxiao Duan Lijie Zhu Youming Zhang Yan Li Fuxiao Liu; This work was supported by the National Natural Science Found ation of China(32273000);the Qingdao Demonstration Project for People-benefit from Science and Techniques,China(23-2-8-xdny-14nsh and 24-2-8-xdny-4-nsh);the National Program of Undergraduate Innovation and Entrepreneurship,China(202310435039);the Open Project Fund of State Key Laboratory of Microbial Technology,China(M2023-03)。; Senecavirus A(SVA)has a positive-sense,single-stranded RNA genome.Its 5´untranslated region harbors an internal ribosome entry site(IRES),comprising 10 larger or smaller stem-loop structures(including a pseudoknot)th...; 关键词：SVA HCV IRES HCV-like IRES stem-loop structure cDNA clone virus rescue mutation

Accurate quantification of 3'-terminal 2'-O-methylated small RNAs by utilizing oxidative deep sequencing and stem-loop RT-qPCR被引量：2: 《Frontiers of Medicine》2022年第2期240-250,共11页Yan Kong Huanhuan Hu Yangyang Shan Zhen Zhou Ke Zen Yulu Sun Rong Yang Zheng Fu Xi Chen; This work was supported by the Fundamental Research Funds for the Central Universities(No.020814380146);National Basic Research Program of China(973 Program)(No.2014CB542300);National Natural Science Foundation of China(Nos.32022015,32001077,31871295,21877060,81250044,81602697,and 81772727);Research Unit of Extracellular Non-Coding RNA,Chinese Academy of Medical Sciences(No.2021RU015).; The continuing discoveries of novel classes of RNA modifications in various organisms have raised the need for improving sensitive,convenient,and reliable methods for quantifying RNA modifications.In particular,a subs...; 关键词：small RNAs 2'-0-methylation SEQUENCING RT-QPCR

棉花miRNA表达量检测方法Stem-loop RT-PCR的建立被引量：2: 《核农学报》2017年第11期2121-2127,共7页胡广王乐张振楠唐叶周璐璐彭清忠吴家和; 国家自然科学基金(31471544;31460066); 为建立一套鉴定棉花miRNA表达丰度的分子技术,优化了Stem-loop RT-PCR方法,并通过分析棉花3个miRNA的表达谱来评价分析该技术体系的特性。首先设计3条引物(茎环特异性反转录引物、正向引物和通用反向引物),通过cDNA和RNA梯度稀释,分析...; 关键词：STEM-LOOP RT-PCR 棉花 MIRNA 茎环引物

Lirex： A Package for Identification of LongInverted Repeats in Genomes: 《Genomics, Proteomics & Bioinformatics》2017年第2期141-146,共6页Yong Wang Jiao-Mei Huang; supported by the National Natural Science Foundation of China (Grant Nos. 41476104 and 31460001);the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB06010201);the International Science and Technology Cooperation Project of Hainan Province, China (Grant No. KJHZ2015-22);the support from the Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences (Grant Nos. SIDSSE-BR-201303 and SIDSSE-201305); Long inverted repeats(LIRs) are evolutionarily and functionally important structures in genomes because of their involvement in RNA interference, DNA recombination, and gene duplication. Identification of LIRs is high...; 关键词：Lirex Inverted repeats Recombination STEM-LOOP Mismatch rate

miRNA qPCR检测方法研究进展及其应用被引量：4: 《生物技术通报》2016年第2期59-69,共11页冯世鹏; 国家自然科学基金项目(31460178);海南省自然科学基金项目(314042);海南省教育厅项目(HNKY2014-16);海南大学科研启动项目(kyqd1306); miRNA是目前国际研究的热点领域之一,而miRNA表达检测是miRNA研究的基础内容。针对miRNA序列短小的特点,开发出了多种检测方法,其中miRNA qPCR定量检测是应用最广泛的方法。就miRNA抽提,miRNA qPCR定量检测原理及miRNA qPCR定量检测流...; 关键词：MIRNA STEM-LOOP RT-PCR poly(A)加尾法RT-PCR 延伸法RT-PCR 数字PCR

Comparison of Two MicroRNA Quantification Methods for Assaying MicroRNA Expression Profiles in Wheat(Triticum aestivum L.)被引量：1: 《Journal of Integrative Agriculture》2014年第4期733-740,共8页HAN Ran YAN Yan ZHOU Peng ZHAO Hui-xian; the National Natural Science Foundation of China(30871578); Two microRNA （miRNA） quantification methods, namely, poly（A） reverse transcription （RT）-quantitative real-time polymerase chain reaction （qPCR） and stem-loop RT-qPCR, have been developed for quantifying miRNA ...; 关键词：Triticum aestivum L. MICRORNA deep sequencing poly（A） RT-qPCR stem-loop RT-qPCR

Rethinking quasispecies theory: From fittest type to cooperative consortia: 《World Journal of Biological Chemistry》2013年第4期79-90,共12页Luis P Villarreal Guenther Witzany; Recent investigations surprisingly indicate that single RNA "stem-loops" operate solely by chemical laws that act without selective forces, and in contrast, self-ligated consortia of RNA stem-loops operate by biologic...; 关键词：QUASISPECIES RNA stem-loops INFECTIOUS AGENTS COOPERATIVE interactions Evolution

不同品种猪肌肉组织miR-1和miR-133基因的表达分析被引量：14: 《畜牧兽医学报》2012年第6期843-848,共6页习欠云周莲莲李虹仪韩东束刚王松波高萍朱晓彤王修启江青艳张永亮; 国家自然科学基金资助(31072105);广东省自然科学基金(10451064201005460);华南农业大学校长基金(2007K005); 本研究旨在探讨猪miR-1和miR-133基因在不同品种猪肌肉组织中的表达情况。试验以180日龄的蓝塘猪、长白猪以及大花白猪的背最长肌为材料,采用Stem-loop实时定量RT-PCR法检测miR-1和miR-133在长白猪、大花白猪及蓝塘猪背最长肌组织中的...; 关键词：STEM-LOOP RT-PCR 肉质性状 MIR-1 miR-133 表达分析

Identification of miR414 and Expression Analysis of Conserved miRNAs from Stevia rebaudiana被引量：4: 《Genomics, Proteomics & Bioinformatics》2011年第6期211-217,共7页Praveen Guleria Sudesh Kumar Yadav; MicroRNAs （miRNAs） usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators. Applications of various computational approaches have simplified the task by predicting miRNAs f...; 关键词：STEVIA MIRNA computational prediction expression analysis target prediction stem-loop reverse transcription

Amplification of unknown RNAs and RNA mixtures based on unique restriction enzyme cleavage in vitro: 《Acta Biochimica et Biophysica Sinica》2010年第12期873-882,共10页Fangyi Yang Jie Wang Yajun Ji Hao Cheng Junting Wan Zhiyu Xiao Guochun Zhou; Small RNAs, generally expressed at low levels, are difficult to reach usable levels from limited material. In this study, we have developed a novel method to amplify target RNA. The amplification procedure was carried...; 关键词：RNA amplification stem-loop linker unknown RNA RNA mixture

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