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Complex relationship between childhood obesity and the gut microbiota: 《World Journal of Clinical Pediatrics》2025年第2期214-220,共7页Trung Nguyen Tran Thang Viet Luong Nam Van Duc Nguyen Hai Nguyen Ngoc Dang; Recently,the gut microbiota has been identified as a significant risk factor associated with metabolic disorders related to obesity.Advances in high-throu-ghput sequencing technology have clarified the relationship be...; 关键词：16S rRNA sequencing Bacterial composition Childhood obesity Gut microbiota FIRMICUTES BACTEROIDETES

In vivo consequences of varying degrees of OTOA alteration elucidated using knock-in mouse models and pseudogene contamination-free long-read sequencing: 《Genes & Diseases》2025年第3期88-92,共5页Ju Ang Kim Bong Jik Kim Chung Lee Go Hun Seo Hane Lee Jin Hee Han Ava Niazi Joosang Park Byung Yoon Choi Sungjin Park; supported by the National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)(No.2021R1C1C1007980 to B.J.K.);Chungnam National University Sejong Hospital Research Fund,2022,and Chungnam National University(to B.J.K.);supported by the Basic Science Research Program through the NRF,funded by the Ministry of Education(No.2021R1A2C2092038 to B.Y.C.);Bio Core Facility Center program(No.NRF-2022M3A9G1014007 to B.Y.C.);the Basic Research Laboratory program through the NRF,funded by the Ministry of Education(No.RS-2023-0021971031482092640001 to B.Y.C.);the Technology Innovation Program(No.K_G012002572001 to B.Y.C.)funded By the Ministry of Trade,Industry&Energy(MOTIE,Korea);funded by SNUBH(Seoul National University Bundang Hospital)intramural research fund(No.13-2022-0010,02-2017-0060,16-2023-0002,13-2023-0002,16-2022-0005,13-2024-0004,and 13-2017-0013 to B.Y.C.);supported by the National Institute on Deafness and Other Communication Disorders(NIDCD);part of the US National Institutes of Health(No.R01DC018814 to S.P.).; Otoancorin(OTOA)is a glycosylphosphatidylinositol(GPI)-anchored protein mediating the attachment of the tectorial membrane(TM)to the spiral limbus(SL)in the inner ear.Homozygous or compound heterozygous mutations in O...; 关键词：vivo consequences glycosylphosphatidylinositol anchored protein autosomal recessive deafness dfnb we tectorial membrane tectorial membrane tm pseudogene contamination free long read sequencing knock mouse models sensorineural hearing lossidentifying

Antibiotic-Depleted Lung Microbiota Modulates Surfactant Proteins Expression and Reduces Experimental Silicosis: 《Biomedical and Environmental Sciences》2025年第4期469-483,共15页Qiang Zhou Meiyu Chang Ning Li Yi Guan Sanqiao Yao; supported by the National Natural Science Foundation of China Joint Fund for Regional Innovation and Development(Grant numbers [U21A20334]);the Postgraduate Innovation Funding Project of Hebei Province(Grant numbers [CXZZBS2022116])。; Objective Recent studies have overturned the traditional concept of the lung as a “sterile organ” revealing that pulmonary microbiota dysbiosis and abnormal surfactant proteins(SPs) expression are involved in the pr...; 关键词：16s rRNA sequencing FIBROSIS Lung microbiota ANTIBIOTICS Surfactant proteins

Prevalence and Molecular Diagnosis of Viruses Infecting Fig Trees in Saudi Arabia: 《Phyton-International Journal of Experimental Botany》2025年第3期897-910,共14页Muhammad Zaman Mahmoud A.Amer Muhammad Amir Zaheer Khalid Muhammad Taimoor Shakeel Ibrahim M.Al-Shahwan Mohammed A.Al-Saleh; The common fig(Ficus carica L.),Moraceae family,is a commonly grown fruit tree in the Mediterranean region.Fig mosaic disease(FMD)poses a substantial threat to Saudi Arabia’s fig-producing economy.A survey was conduc...; 关键词：FIG FMD viruses GRAFTING RT-PCR SEQUENCING

Traditional Chinese medicine and modern technology:Network pharmacology and omics sequencing in gastric cancer: 《World Journal of Gastrointestinal Oncology》2025年第3期38-43,共6页Jessica Shapiro Gemmell Brandon Lucke-Wold; In this editorial,we comment on the article by Micucci et al published in the recent issue.We focus on the heterogenous nature of gastric cancer(GC)and the potential benefits of integrating traditional Chinese medicin...; 关键词：Gastric cancer Traditional Chinese medicine OMICS Network pharmacology NUTRACEUTICALS Precision medicine

Checkpoint kinase 1 in colorectal cancer:Upregulation of expression and promotion of cell proliferation: 《World Journal of Clinical Oncology》2025年第3期95-115,共21页Yu-Yan Pang Zu-Yuan Chen Da-Tong Zeng Dong-Ming Li Qi Li Wan-Ying Huang Bin Li Jia-Yuan Luo Bang-Teng Chi Qiu Huang Zhen-Bo Feng Rong-Quan He; Supported by Innovation Project of Guangxi Graduate Education,No.YCBZ2023096;Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z20210442;China Undergraduate Innovation and Entrepreneurship Training Program,No.S202410598185;Future Academic Star of Guangxi Medical University,No.WLXSZX24101.; BACKGROUND Colorectal cancer(CRC)is a prevalent malignant tumor characterized by a high mortality rate,with significant challenges persisting in the identification and management of its metastatic stage.The role of ch...; 关键词：Colorectal cancer Checkpoint kinase 1 Single-cell sequencing IMMUNOHISTOCHEMISTRY Molecular docking

Comprehensive cultivation of the broiler gut microbiota guides bacterial isolation from chickens: 《Science China(Life Sciences)》2025年第3期836-845,共10页Zhang-Chao Deng Ke-Xin Cao Yu-Xuan Huang Zhe Peng Ling Zhao Dan Yi Meng Liu Lv-Hui Sun; supported in part by the National Key Research and Development Program of China Projects(2022YFD1300402);fundamental research funds for the Central Universities(2662022DKPY002);the Top-Notch Young Talent Supporting Program(to LH Sun).; Chicken gut microbiota plays an important role in maintaining their physiological health.However,the cultivability of chicken gut microbiota is not well understood,limiting the exploration of certain key gut bacteria ...; 关键词：CHICKEN gut microbes culturomics 16S rRNA sequencing lactic acid bacteria

Single-cell transcriptomic sequencing identifies subcutaneous patient-derived xenograft recapitulated medulloblastoma: 《Animal Models and Experimental Medicine》2025年第3期458-472,共15页Jiayu Gao Yahui Zhao Ziwei Wang Fei Liu Xuan Chen Jialin Mo Yifei Jiang Yongqiang Liu Peiyi Tian Yanong Li Kaiwen Deng Xueling Qi Dongming Han Zijia Liu Zhengtao Yang Yixi Chen Yujie Tang Chunde Li Hailong Liu Jiankang Li Tao Jiang; National Key Research and Development Program of China,Grant/Award Number:2022ZD0210100;Beijing Nova Star Program,Grant/Award Number:2022002;Natural Science Foundation of Beijing and Haidian Collaboration Foundation,Grant/Award Number:L232079;National Natural Science Foundation of China,Grant/Award Number:82172608,82273343,81902975 and 82101356;Capital Medical University Fund for Excellent Young Scholars,Grant/Award Number:KCB2304。; Background:Medulloblastoma(MB)is one of the most common malignant brain tumors that mainly affect children.Various approaches have been used to model MB to facilitate investigating tumorigenesis.This study aims to com...; 关键词：experimental models MEDULLOBLASTOMA single-cell sequencing sPDX

Toward understanding the role of genomic repeat elements in neurodegenerative diseases: 《Neural Regeneration Research》2025年第3期646-659,共14页Zhengyu An Aidi Jiang Jingqi Chen; supported by the National Natural Science Foundation of China, No.61932008;Natural Science Foundation of Shanghai, No.21ZR1403200 (both to JC)。; Neurodegenerative diseases cause great medical and economic burdens for both patients and society;however, the complex molecular mechanisms thereof are not yet well understood. With the development of high-coverage se...; 关键词：Alzheimer's disease ATAXIA deep learning long-read sequencing NEURODEGENERATION neurodegenerative diseases Parkinson's disease repeat element structural variant

Recent technology advancements in large-scale DNA assembly: 《Quantitative Biology》2025年第1期143-151,共9页Jielin Li Li Cheng Yingxin Ma Yizhi Cai Junbiao Dai; Elite Young Scientists Program of CAAS;National Natural Science Foundation of China,Grant/Award Numbers:32150025,31725002;Guangdong Provincial Key Laboratory of Synthetic Genomics,Grant/Award Number:2023B1212060054;Shenzhen Outstanding Talents Training Fund;Science and Technology Planning Project of Guangdong Province,Grant/Award Number:2022A0505090009;Bureau of International Cooperation,Chinese Academy of Sciences,Grant/Award Number:172644KYSB20180022;Innovation Program of Chinese Academy of Agricultural Sciences;Shenzhen Science and Technology Program,Grant/Award Number:KQTD20180413181837372;National Key Research and Development Program of China,Grant/Award Number:2022YFF1201800;Shenzhen Key Laboratory of Synthetic Genomics,Grant/Award Number:ZDSYS201802061806209。; The emergence and development of sequencing and DNA synthesis techniques have empowered us with the ability to read and write genomes,thereby enhancing our understanding and manipulation of biological processes.Over t...; 关键词：saccharomyces cerevisiaewith synthetic yeast genome sequencing dna synthesis techniques understanding manipulation biological processesover sequencing techniques constructed all chromosomes read write genomesthereby artificially synthesized notablythe

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