Prevalence and Molecular Diagnosis of Viruses Infecting Fig Trees in Saudi Arabia  

作　　者：Muhammad Zaman Mahmoud A.Amer Muhammad Amir Zaheer Khalid Muhammad Taimoor Shakeel Ibrahim M.Al-Shahwan Mohammed A.Al-Saleh 

机构地区：[1]Plant Protection Department,College of Food and Agriculture Sciences,King Saud University,Riyadh,145111,Saudi Arabia [2]Department of Plant Pathology,Faculty of Agriculture and Environment,The Islamia University of Bahawalpur,Bahawalpur,63100,Pakistan [3]Chair of Date Palm Research,Center for Chemical Ecology and Functional Genomics,College of Food and Agriculture Sciences,King Saud University,Riyadh,11451,Saudi Arabia

出　　处：《Phyton-International Journal of Experimental Botany》2025年第3期897-910,共14页国际实验植物学杂志(英文)

摘　　要：The common fig(Ficus carica L.),Moraceae family,is a commonly grown fruit tree in the Mediterranean region.Fig mosaic disease(FMD)poses a substantial threat to Saudi Arabia’s fig-producing economy.A survey was conducted during the two seasons of 2021 and 2022 on fig trees,displaying various fig mosaic disease symptoms.A total of 200 fig leaves and fruit samples were collected from various governorates in several Regions of Saudi Arabia including Riyadh,Tabuk,Hail,Qassim,Al-Jouf,Makkah,Jazan,Al-Madinah,Asir,and Northern Borders.These samples were tested by reverse transcription polymerase chain reaction(RT-PCR)with specific pairs of primers to assess the existence of Fig mosaic virus(FMV),Fig leaf mottle-associated virus 1(FLMaV-1),Fig leaf mottle-associated virus 2(FLMaV-2),Fig mild mottle-associated virus(FMMaV)and Fig fleck-associated virus(FFkaV).The results indicate that four viruses were found in mixed infections and tested positive.FMV was detected with a high infection rate of 46%followed by FLMaV-2 with an infection rate of 20%,FMMaV with an infection rate of 16%,and FLMaV-1 with an infection rate of 7%,respectively,while FFkaV was negative in all tested samples.Sequencing and phylogenetic analysis results showed that the FMV isolates shared 90.3%similarity with other FMV isolates,particularly those from Bosnia and Herzegovina(KU198368).While FLMaV-1 showed 92.5%similarity with the reference isolate FLMaV-1(KX397035),the isolates of FLMaV-2 exhibited 94.7%similarity with reference FLMaV-2 isolate(FN687742),and the isolate of FMMaV showed 94%similarity with reference FMMaV isolate(MG242131)based on sequence comparison.According to the RT-PCR results,FMV was effectively identified in all five fig varieties(Al Faiz Yellow,Asali,Brown Turkey,Iraqi,and Kaab Al-Ghazal).Contrarily,none of these varieties had FLMaV-1,FLMaV-2,or FMMaV.This study investigates the occurrence and economic impact of the viruses that infect fig trees in Saudi Arabia.This suggests that FMV is the primary virus infecting these fig vari

关 键 词：FIG FMD viruses GRAFTING RT-PCR SEQUENCING 

分 类 号：R51[医药卫生—内科学]