咪达普利对氯化铵所致急性肺损伤大鼠血气、MDA及Ang Ⅱ、CD54蛋白表达的影响  

Effects of imidapril on blood gas,oxidative stress and inflammatory factors in rats with acute lung injury induced by ammonium chloride

作　　者：黄丛富 郝雪琴[1] 邓雯[1] 刘传发 王婷婷[1] 温小利[1] 陶中蓉 

机构地区：[1]河南科技大学动物科技学院药学系,河南洛阳471003

出　　处：《中国病理生理杂志》2016年第2期278-283,共6页Chinese Journal of Pathophysiology

摘　　要：目的:本研究通过复制氯化铵致大鼠肺损伤模型,研究咪达普利对肺损伤大鼠动脉血气、MDA、肺形态学和AngⅡ、CD54蛋白表达的影响。方法:将30只雄性大鼠随机分为3组:空白对照组、肺损伤模型组和药物组。对照组大鼠腹腔注射生理盐水2 mL/kg;肺损伤模型组大鼠腹腔注射等量6%氯化铵;药物组大鼠在注射6%氯化铵后,每天1次灌胃咪达普利(3 mg/kg),连续灌胃7 d;在实验第7天,用2%的戊巴比妥钠麻醉,采集腹主动脉血和腹腔静脉血样本;肺组织经4%多聚甲醛灌注、固定、脱水、透明、浸蜡、包埋和切片。ELISA法测定血清TNF-α和IL-6水平,化学发光法测定血清MDA含量,HE染色观察肺部形态学变化,免疫组织化学染色法观察肺组织AngⅡ和CD54蛋白表达。结果:与对照组相比,模型组PaCO_2明显升高(P<0.05),血清TNF-α、IL-6和MDA浓度显著增加(P<0.01),氯化铵腹腔注射的肺损伤模型组大鼠出现肺水肿、肺炎、肺泡壁瘀血、肺泡腔出血和肺泡壁增生等损伤,肺组织AngⅡ和CD54蛋白表达明显增高(P<0.01);与模型组比较,药物组PaCO_2明显降低(P<0.05),血清TNF-α、IL-6和MDA浓度以及肺组织AngⅡ、CD54蛋白表达显著降低(P<0.01)。结论:咪达普利可显著改善氯化铵所致肺损伤大鼠血气,抑制脂质过氧化和血清炎症细胞因子,降低肺组织AngⅡ和CD54蛋白表达。AIM： In this study, the rat lung injury model was induced by ammonium chloride for studying the effect of imidapril on blood gas, serum TNF-α, IL-6 and MDA concentrations, and Ang Ⅱ and CD54 protein expression in rat lung tissue. METHODS： Male rats were randomly divided into 3 groups： control group, lung injury model group and drug group. The rats in control group were given saline （2 mL/kg） , while the rats in lung injury model group were given 6% ammonium chloride （2 mL/kg）. In drug group, imidapril （3 mg · kg^-1 · d^-1） was given to the rats once daily for 1 week by intragastric gavage after given 6% ammonium chloride. On the 7th day, the rats were anesthetized with 2% so- dium pentobarbital. Abdominal aorta blood, venous blood and lung tissue were collected. The blood gas indexes and serum TNF-α, IL-6 and MDA concentrations were determined. The lung tissues were fixed and sliced, and the expression of Ang Ⅱ and CD54 proteins was deteeted by immunohistoehemistry. RESULTS： The PaCO2 increased in lung injury model group compared with control group and drug group （ P 〈 0. 05 ）. The expression of Ang Ⅱ and CD54, and the concentrations of TNF-α, IL-6 and MDA also inereased significantly （ P 〈 0. 01 ） in model group. Pulmonary edema, inflammation, alveo- lus congestion, hemorrhage and hyperplasia in model group were obvious compared with control group and drug group. CONCLUSION： Imidapril improves blood gas indexes, and reduces lipid peroxidation and inflammatory responses in the rats with lung injury induced by ammonium chloride.

关 键 词：咪达普利 急性肺损伤 氯化铵 

分 类 号：R363[医药卫生—病理学]