肝细胞癌中microRNA-375调控的基因网络分析  

Analysis of gene network regulated by microRNA-375 in HCC

作　　者：黄波[1] 肖影群[1] 罗达亚[2] 章萍[1] 杨仙荷[1] 钟青梅[1] 王武[1] 姚迪[1] 

机构地区：[1]南昌大学附属感染病医院病理科,江西南昌330002 [2]南昌大学基础医学院生物化学与分子生物学教研室,江西南昌330006

出　　处：《中国病理生理杂志》2016年第2期363-370,共8页Chinese Journal of Pathophysiology

基　　金：2011江西省卫生厅科技计划(No.20112043);2012年南昌市市校合作项目(洪财企[80]号)

摘　　要：目的:探讨肝细胞癌中miR-375的表达及其调控的相关靶基因和信号通路。方法:采用原位杂交检测miR-375在人肝癌组织芯片的表达情况;人全基因组表达谱芯片检测4株肝癌细胞株;MAS生物信息学软件筛选miR-375调控靶基因及相关信号通路。结果:原位杂交结果显示miR-375在肝癌组织中的表达明显高于癌旁组织(P<0.05);人全基因组表达谱芯片结果分析显示4株转染miR-375的肝癌细胞的共上调基因有20个,共下调基因有17个;MAS生物信息学软件分析显示4株转染miR-375的肝癌细胞共有的上调信号通路有54条,下调信号通路有48条。结论:miR-375与肝细胞癌有密切的关系。对miR-375调控的肝细胞癌相关靶基因与信号通路进行多元化筛选,为后续全面深入的研究肝细胞癌发生发展的机制提供了便利。AIM： To investigate the expression of microRNA-375 （miR-375） in hepatocellular carcinoma （HCC） and to analyze the target genes and signaling pathways regulated by miR-375. METHODS： The expression of miR-375 was examined at tissue microarray of HCC by in situ hybridization. The whole human genome chip and bioinforma- tics analysis were applied to screen out the differential expression genes and signaling pathways in 4 HCC cell lines trans- fected with miR-375 mimic. RESULTS ： In situ hybridization showed the expression of miR-375 in HCC tissues were obvi- ously higher than that in tumor-adjacent tissues （ P 〈 0. 05 ）. There were 20 co-upregulated genes and 17 co-downregulated genes in all 4 cell lines. Bioinformatic analysis showed that there were 54 signaling pathways related to up-regulated genes and 48 signaling pathways related to down-regulated genes in all 4 cell lines. CONCLUSION： miR-375 may play a key role in the pathological process of HCC. The bioinformatic analysis is able to screen the target genes and signaling pathways regulated by miR-375 and to provide an explicit direction for further mechanism research on HCC.

关 键 词：肝细胞癌 MicroRNA-375 原位杂交 基因芯片 

分 类 号：R730.23[医药卫生—肿瘤] R735.7[医药卫生—临床医学]