低聚古罗糖醛酸磷酸酯的制备及其对RAW264.7细胞的激活作用  

The preparation of low-molecular-weight polyguluronate phosphate and its immunostimulatory activity on RAW264.7 cell

作　　者：李全才[1] 辛萌[1] 杨程[1] 李海花[1] 管华诗[1] 李春霞[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2016年第1期29-34,共6页Chinese Journal of Marine Drugs

基　　金：海洋公益性行业科研专项基金项目(201405038);国家自然科学基金委员会-山东省人民政府联合资助海洋科学研究中心项目(U1406402);青岛市自主创新重大专项(13-7-1-zdzx1-hy)资助

摘　　要：目的研究低聚古罗糖醛酸磷酸酯对RAW264.7细胞的激活作用。方法低聚褐藻胶依次通过pH分级、高压酸解纯化的方法制备低聚古罗糖醛酸,进而采用磷酸/尿素法对其进行磷酸化修饰,制备低聚古罗糖醛酸磷酸酯。在低聚古罗糖醛酸磷酸酯对RAW264.7细胞激活实验中,分别测定了对RAW264.7细胞增殖、吞噬能力、细胞因子(TNF-α、IL-1β)释放、胞内酶(酸性磷酸酶、乳酸脱氢酶)活力的影响。结果低聚古罗糖醛酸磷酸酯能显著促进RAW264.7细胞的增殖,提高其对中性红的吞噬,促进细胞因子的释放以及增强胞内酶的活性。结论低聚古罗糖醛酸磷酸酯能够多层次、多水平的激活RAW264.7细胞,是1种潜在的免疫增强剂。Objective To investigate the immunostimulatory effect of low-molecular-weight polyguluronate phosphate on RAW264.7cell.Methods Low-molecular-weight polyguluronate（LPG）was obtained after the pH fractionation and acid hydrolysis of low-molecular-weight alginate,and then H3PO4/Urea method was used to afford low-molecular-weight polyguluronate phosphate（LPGP）.In the immunostimulatory assay,cells proliferation,phagocytosis,cytokines（TNF-α,IL-1β）release and activities of intracellular enzymes（acid phosphatase,lactate dehydrogenase）were determined to study the effects of LPGP on RAW264.7cell.Results LPGP could significantly improve the cell proliferation,the neutral red phagocytosis,cytokines release and intracellular enzymes activities of RAW264.7cell.Conclusion LPGP could effectively activate RAW264.7cell via multiple levels.It indicated that LPGP was a potential immunoenhancer.

关 键 词：低聚古罗糖醛酸 磷酸酯 RAW264.7细胞 免疫激活 

分 类 号：R914[医药卫生—药物化学] R965[医药卫生—药学]