HPV16 E6对Hippo途径效应分子YAP表达和定位的影响  

Effect of HPV16 E6 Protein on the Expression and Localization of Effector YAP in the Hippo Pathway

作　　者：龙洪清[1] 向赟[2] 张振[2] 郑义[3] 

机构地区：[1]湖北科技学院临床医学院肿瘤学与核医学教研室,湖北咸宁437100 [2]武汉市妇女儿童医疗保健中心,湖北武汉430016 [3]南方医科大学附属广州花都区人民医院中心实验室,广东广州510800

出　　处：《肿瘤学杂志》2016年第2期125-128,共4页Journal of Chinese Oncology

基　　金：湖北省卫生厅科研一般项目(JX6B97)

摘　　要：[目的]研究HPV16 E6和Hippo途径效应分子YAP的相关性。[方法]用Western Blot、RT-PCR和激光共聚焦显微镜观察HPV16 E6过表达或敲减后YAP的表达和定位,以及其直接作用的下游基因结缔组织生长因子(CTGF)的表达。[结果]HPV16 E6敲减或过表达时YAP相应地表达减少或增加,磷酸化的YAP增加或减少,YAP主要定位于胞浆或胞浆和胞核中,YAP下游CTGF的表达相对应减少或增加。[结论]HPV16 E6诱导Hippo途径失常,YAP的表达升高,可能提升HPV16 E6的致癌性,Hippo途径可能是治疗高危性HPV感染相关疾病的候选药物作用靶标。[Purpose] To explore the association between HPV16 E6 and Yap. [Methods] The expression and localization of YAP and its downstream connective tissue growth factor（CTGF） were analyzed using Western Blot,RT-PCR and laser scanning confocal microscope after HPV16 E6 overexpression and knockdown. [Results] HPV16 E6 knockdown or overexpression induced the decreasing or increasing YAP expression,and Yap mainly located in cytoplasm or cytoplasm and nuclear,and CTGF expression decreased or increased. [Conclusion] HPV16 E6 induces Hippo pathway deregulation and Yap overexpression might promote HPV16 E6 pathogenesis,implying that Hippo pathway might be candidate drug target of hr-HPV infection-induced diseases.

关 键 词：人乳头瘤病毒 E6蛋白 Hippo信号途径 YAP 

分 类 号：R73[医药卫生—肿瘤]