A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles  

作　　者：Xia Li Juan Song Bao-Li Chen Bing Wang Rui Li Hui-Min Jiang Ji-Feng Liu Chen-Zhong Li 

机构地区：[1]Department of Chemistry, Liaocheng University,Liaocheng 252059, China [2]Key Laboratory of Food Nutrition and Safety, Ministry ofEducation, Tianjin University of Science and Technology,Tianjin 300457, China [3]Nanobioengineering/Bioelectronics Laboratory, Department ofBiomedical Engineering, Florida International University,Miami, FL 33174, USA

出　　处：《Science Bulletin》2016年第4期276-281,共6页科学通报（英文版）

基　　金：the National Natural Science Foundation of China (21305058, 21205056, 21075058 and 21503104);Tai-Shan Scholar Research Fund of Shandong Province

摘　　要：A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles(PNA/Ag NPs) has been initially proposed for specific recognition of m RNA.Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc m RNA detection can be achieved. Moreover, the PNA/Ag NPs platform can undergo color change in response to target c-Myc m RNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles （PNA/AgNPs） has been initially proposed for specific recognition of mRNA. Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA-RNA complex, proto-oncogene c-Myc mRNA detection can be achieved. Moreover, the PNA/AgNPs platform can undergo color change in response to target c-Myc mRNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.

关 键 词：Colorimetric · c-Myc mRNA · Peptidenucleic acid · Silver nanoparticles 

分 类 号：R730.43[医药卫生—肿瘤] O657.3[医药卫生—临床医学]