肺癌患者血清趋化因子的表达水平及临床意义  

Expression and clinical significance of serum chemokines in patients with lung cancer

作　　者：王菀菀 孙俊宁[1] 操珍[1] 李海燕[1] 苏文[1] 

机构地区：[1]山西医科大学附属肿瘤医院免疫室,太原030013

出　　处：《国际肿瘤学杂志》2016年第2期90-94,共5页Journal of International Oncology

基　　金：山西省卫生厅科技攻关基金(2011048) Science and Technology Project Fund of Shanxi Provincial Health Department

摘　　要：目的 检测干扰素诱导的T细胞趋化因子（ITAC）、Fractalkine、巨噬细胞炎性蛋白（MIP）-3α、白细胞介素8（IL-8）、MIP-1α和MIP-1β在肺癌患者血清的表达水平,分析其与肺癌临床特征之间的关系及各趋化因子间相关性.方法 采用液相芯片技术联合检测40例初诊肺癌患者和30例正常人血清中上述6种趋化因子的表达情况,采用SPSS 17.0软件分析其与肺癌临床特征的关系及各趋化因子之间的相关性.结果 肺癌患者血清IL-8、Fractalkine、MIP-3α表达量中位数（四分位数间距）分别为5.16（4.74）、128.45 （141.89）、10.31（8.88）,正常人分别为2.01（0.95）、61.46（74.81）、8.08（5.87）,组间差异均有统计学意义（Z=-4.783,P ＜0.001;Z=-4.046,P＜0.001;Z=-3.105,P=0.002）.肺腺癌组MIP-1β水平显著高于鳞状细胞癌组[18.32（12.27）∶13.72（7.31）,Z=-2.212,P =0.027],而鳞状细胞癌组ITAC水平显著高于小细胞肺癌组[24.51（22.48）∶9.28（4.85）,Z=-2.460,P=0.014];在肺癌患者和正常人中,MIP-3α与Fractalkine均呈正相关（r=0.619,P ＜0.001;r=0.766,P ＜0.001）.结论 IL-8 、Fractalkine、MIP-3α在肺癌患者中的表达明显升高,可能在肺癌的转移中发挥重要作用.Objective To detect the expression levels of multiple serum chemokines including IFN-inducible T cell chemoattractant （ITAC）,Fractalkine,macrophage inflammatory protein （MIP）-3α,IL-8,MIP-lα,MIP-1β in patients with lung cancer and explore their association with the clinical characteristics of lung cancer as well as the correlations among these chemokines.Methods Forty newly diagnosed patients with lung cancer and thirty healthy controls were enrolled for detection of the serum levels of 6 kinds of chemokines by Luminex technology.The correlations of clinical characteristics of lung cancer with these chemokines and the correlations among these chemokines were analyzed by SPSS 17.0 software.Results The serum levels [M （QR）] of IL-8,Fractalkine and MIP-3α in patients with lung cancer were 5.16 （4.74）,128.45 （141.89）,10.31 （8.88） respectively,and 2.01 （0.95）,61.46 （74.81）,8.08 （5.87） respectively in control group,with significant differences （Z =-4.783,P 〈0.001;Z =-4.046,P 〈0.001;Z =-3.105,P =0.002）.The expression of MIP-1β in lung adenocarcinoma was significantly higher than that in squamous carcinoma [18.32 （12.27） vs.13.72 （7.31）,Z =-2.212,P =0.027],and of ITAC in squamous carcinoma was significantly higher than that in small cell lung cancer [24.51 （22.48） vs.9.28 （4.85）,Z =-2.460,P =0.014].The expressions of MIP-3α and Fractalkine were positively correlated in the two groups （r =0.619,P〈0.001;r=0.766,P〈0.001）.Conclusion The expressions of IL-8,Fractalkine and MIP-3α increase significantly in lung cancer patients,and they are may play important roles in metastatic lung cancer.

关 键 词：肺肿瘤 趋化因子类 受体 趋化因子 

分 类 号：R734.2[医药卫生—肿瘤]