人参皂甙Rg3对前列腺癌细胞的作用研究  

Effects of ginsenoside Rg3 on prostate cancer cells

作　　者：尹怀福[1] 修有成[1] 赵维明[1] 金承俊[1] 张子健[1] 

机构地区：[1]哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001

出　　处：《现代医学》2016年第1期1-6,共6页Modern Medical Journal

基　　金：国家自然科学基金资助项目(81170534)

摘　　要：目的:通过研究人参皂甙Rg3(GS-Rg3)对前列腺癌LNCaP细胞增殖和凋亡的作用及其相关分子生物学机制,为前列腺癌的药物治疗提供新的方向。方法:分别用不同浓度的GS-Rg3作用于LNCa P细胞48 h,通过MTT法及流式细胞仪检测LNCa P细胞增殖及凋亡的情况;利用RT-PCR技术检测LNCaP细胞凋亡相关基因caspase-3、Bcl-2及前列腺标志基因雄激素受体(AR)、5α还原酶(5α-R)及前列腺特异性抗原(PSA)的表达。结果:(1)GS-Rg3对前列腺癌细胞系LNCaP的增殖有明显的抑制作用,而且呈剂量依赖性;(2)GS-Rg3可以诱导LNCa P细胞剂量依赖性的凋亡,该凋亡与增强caspase-3及抑制Bcl-2基因表达相关;(3)GS-Rg3可以抑制LNCaP细胞前列腺标志基因AR、5α-R及PSA的表达。结论:GS-Rg3可以抑制LNCaP细胞增殖,诱导细胞凋亡,对雄激素依赖性LNCa P细胞具有多重抗癌作用。Objective： To investigate the effect and mechanism of ginsenoside Rg3（ GS-Rg3） on the proliferation and apoptosis of human prostate cancer cell line LNCa P in order to provide an understanding for developing of GSRg3 on the treatment of prostate cancer. Methods： LNCa P cells were cultured for 48 hours by administrating different concentrations of GS-Rg3. Cell viability was measured by MTT and flow cytometer. Cell apoptosis-related genes caspase-3,Bcl-2 and prostate cell marker gene 5α-reductase（ 5α-R）,androgen receptor（ AR）,prostate specific antigen（ PSA） were analyzed by reverse transcription polymerase chain reaction（ PCR）. Results： GS-Rg3 inhibited LNCa P cells proliferation and induced cell apoptosis. The expression of caspase-3 was increased while Bcl-2 was decreased after GS-Rg3 treatment in vitro. GS-Rg3 also exhibited a dose-dependent inhibition on the expression of all three prostate cell marker genes（ AR,5α-R and PSA）. Conclusion： GS-Rg3 shows multiple antitumor effects on prostate cancer by inhibiting LNCa P cells proliferation and inducing its apoptosis.

关 键 词：人参皂甙RG3 前列腺癌LNCaP细胞 凋亡 基因表达 

分 类 号：R737.25[医药卫生—肿瘤]