大豆分离蛋白-乙二胺接枝物的合成及性质  

Synthesis and properties of grafted copolymers based on soy protein isolated with ethylenediamine

作　　者：袁明昆 许楚[1] 周景辉[1] 

机构地区：[1]大连工业大学轻工与化学工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2016年第1期23-28,共6页Journal of Dalian Polytechnic University

基　　金：国家科技支撑计划项目(2013BAC01B03)

摘　　要：探讨了以1-(3-二甲氨基丙基-3)-乙基碳二亚胺盐酸盐(EDC)和N-羟基琥珀酰亚胺(NHS)催化大豆分离蛋白(SPI)的羧基和乙二胺(EDA)的氨基进行接枝反应制备大豆分离蛋白-乙二胺接枝物(SPI-EDA)的最佳条件。利用激光粒度/Zeta电位仪、FT-IR、HPLC、PCD等对SPI-EDA接枝物进行结构与性质表征。确定适宜的合成条件:反应温度为室温,时间4h,SPI、EDC、EDA与NHS摩尔比1.0∶0.5∶0.6∶0.125。检测结果表明,EDA成功的接枝到SPI羧基基团上,与SPI相比,SPI-EDA的电荷密度、相对分子质量等都有所改善,接枝物SPI-EDA聚集体粒径增大且分布均一。Grafted copolymer （SPI-EDA） was prepared by grafting reaction between the free carboxylic acid groups of soy protein isolate （SPI） and the amino groups of ethylenediamine （EDA） using 1-（3-（dimethylamino）propyl）-3-ethyl-carbodiimide hydrochloride （EDC） and N-hydroxysuccinimide （NHS） as the condensing agents. The optimal condition for synthesis of SPI-EDA was as follows： room temperature of reaction temperature, 4 h of reaction time, 1 ： 0.5 ： 0.6 ： 0. 125 molar ratio of SPI, EDC, EDA and NHS. The chemical structure and physical property of SPI-EDA was analyzed by laser particle size/Zeta potential, FT-IR, HPLC and PCD, respectively. The results revealed that EDA was grafted onto the carboxyl group of SPI. The charge density and molecular weight of SPI-EDA were improved compared with SPI, and the size of SPI-EDA aggregates was increased and the distribution was uniform.

关 键 词：大豆分离蛋白(SPI) 1-(3-二甲氨基丙基-3)-乙基碳二亚胺盐酸盐(EDC) 乙二胺(EDA) 大 豆分离蛋白-乙二胺接枝物(SPI-EDA) 

分 类 号：TS727[轻工技术与工程—制浆造纸工程]