阿司匹林对糖尿病大鼠肾缺血再灌注损伤时Cystatin C的影响  

The Effects of Aspirin on Cystatin C in Renal Ischemia-reperfusion Model of Diabetic Rats

作　　者：蒋玮[1] 何建斌[2] 陶蕾[2] 罗斌[2] 姚立农[1] 

机构地区：[1]第四军医大学唐都医院重症监护中心,陕西西安710038 [2]第四军医大学唐都医院麻醉科,陕西西安710038

出　　处：《现代生物医学进展》2016年第3期453-456,共4页Progress in Modern Biomedicine

摘　　要：目的:评价阿司匹林对糖尿病大鼠肾缺血再灌注损伤后Cystatin C(蛋白酶抑制肽C)的影响。方法:32只成年Sprague-Dawley大鼠经链脲霉素(streptozotocin,STZ)腹腔注射建立糖尿病模型后随机分为4组,实验组分别经胃灌注10 mg/kg、20 mg/kg、30 mg/kg的阿司匹林,对照组灌注等量生理盐水15 d后建立肾缺血30 min再灌注2 h模型。抽取动脉血用ELISA法检测Cystatin C水平,取肾脏做病理切片和免疫组化检测。结果:各实验组血清Cystatin C水平明显低于对照组(P<0.05),实验组之间差异不显著(P>0.05)。HE染色实验组与对照组未见明显组织病理学差异。免疫组化显示对照组Cystatin C蛋白表达增多,而实验组表达不显著。结论:低剂量阿司匹林降低STZ诱导的糖尿病大鼠肾缺血再灌注后血浆Cystatin C水平,具有肾保护作用。Objective： To evaluate the effects of aspirin on serum Cystatin C expression after renal ischemia-reperfusion in streptozotocin（STZ） induced diabetic rats. Methods： Thirty two adult male Sprague-Dawley rats were randomized into four groups after diabetic models completed by intraperitoneal injection streptozotocin. Citric buffer solution melted aspirin gavage was administered at doses of 10 mg/kg, 20 mg/kg and 30 mg/kg in each treated group, equivalent physiological saline in control group for 15 d. All rats were exposed to well-established rodent ischemia-reperfusion model for renal pedicle clamped for 30 min and reperfusion 2 h. Blood samples were obtained for ELISA analysis of serum Cystatin C and kidney were harvested for micrography by hematoxylin and eosin stain and immunohistochemical tests. Results： Serum cys-C levels in treated group significantly decreased compared with control group（P〈0.05）,but there are no significant difference within treated groupsno significant differences were observed between treated groups when compared with each other（P〈0.05）. No characteristic changes were obtained in both control and treated groups by HE micrography. Immunohistochemistry implied elevation of Cystatin C protein secretion in control group but moderate in treated groups. Conclusion： Aspirin administration at low dosage decrease serum Cystatin C levels after renal ischemia-reperfusion injury and maybe nephric protective in STZ induced diabetic rats.

关 键 词：阿司匹林 缺血再灌注损伤 急性肾功能损伤 

分 类 号：R-33[医药卫生]