上皮细胞间质化预测胰腺癌预后的价值  

Relationship between epithelial to mesenchymal transition and prognosis in pancreatic cancer

作　　者：马睿锐[1] 贡海兵[1] 龚健[2] 徐彬[2] 

机构地区：[1]上海市松江区中心医院消化外科,上海市201600 [2]同济大学附属第十人民医院普外科,上海市200072

出　　处：《世界华人消化杂志》2016年第5期686-691,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨上皮细胞间质化(epithelial to mesenchymal transition,EMT)与胰腺癌预后的关系.方法:采用免疫组织化学的方法检测60例胰腺癌组织中E-cadherin、Vimentin蛋白的表达,根据E-cadherin和Vimentin的表达将EMT程度分为3级:1级:E-cadherin(+)和Vimentin(-);2级:E-cadherin(-)和Vimentin(-);3级:E-cadherin(-)和Vimentin(+).通过生存分析研究上皮间质化程度与预后的关系.结果:60例胰腺癌患者的1、2、3年累计生存率分别是50%、29%、21%.E-cadherin高表达与胰腺癌较好预后相关(P=0.006);而Vimentin的表达与胰腺癌预后呈负相关(P=0.002).E M T 1级、2级、3级中位生存时间分别为24.07 mo±4.50 mo、11.97 mo±0.92m o、6.30 m o±0.52 m o,上皮细胞-间质化程度越高预后越差(P=0.001).Cox比例风险模型提示EMT程度是影响患者预后的独立因素(P=0.025),其HR值为1.779(95%CI:1.077-2.939).结论:上皮细胞-间质化是胰腺癌预后的独立危险因素,其间质化程度越高,预后越差.AIM： To investigate the relationship between epithelial to mesenchymal transition（EMT） and patient＇s prognosis in pancreatic cancer. METHODS： E-cadherin and Vimentin protein expression was detected by immunohistochemistry in 60 pancreatic cancer specimens. EMT was divided into three grades according to the expression of E-cadherin and Vimentin： Ⅰ [E-cadherin（＋）/ Vimentin（-）], Ⅱ [E-cadherin（-）/Vimentin（-）], and Ⅲ [E-cadherin（-）/Vimentin（＋）]. KaplanMeier survival analysis was used to evaluate the relationship between EMT and patient＇s prognosis. RESULTS： The 1-, 2-, and 3-year overall survival rates in 60 patients with pancreatic cancer were 50%, 29% and 50%, respectively. High expression of E-cadherin was associated with a good prognosis（P = 0.006）, while elevated expression of Vimentin related with a poor prognosis（P = 0.002）. Median survival of patients with grades Ⅰ, Ⅱ and Ⅲ EMT was 24.07 mo ± 4.50 mo, 11.97 mo ± 0.92 mo and 6.30 mo ± 0.52 mo, respectively. A higher grade of EMT was associated with a worse prognosis（P = 0.001）. Cox proportional hazards models suggested that EMT was one of independent risk factors for poor prognosis in pancreatic cancer（P = 0.025）, with HR = 1.779（95%CI： 1.077-2.939）. CONCLUSION ： EMT is an independent predicable risk factor for poor prognosis in pancreatic cancer.

关 键 词：上皮细胞间质化 胰腺癌 独立危险因素 

分 类 号：R735.9[医药卫生—肿瘤]