COLD-PCR/Sanger法检测非小细胞肺癌患者外周血EGFR和KRAS基因突变的价值  

Value of COLD-PCR/Sanger in Detecting EGFR and KRAS Gene Mutations in Peripheral Blood of Patients with Non-Small Cell Lung Cancer

作　　者：周建英[1] 刘震天[1] 朱伟锋[2] 

机构地区：[1]江西省肿瘤医院内二科,南昌330029 [2]南昌大学基础医学院生物化学与分子生物学教研室,南昌330006

出　　处：《南昌大学学报（医学版）》2015年第6期38-41,共4页Journal of Nanchang University：Medical Sciences

摘　　要：目的评价COLD-PCR/Sanger法检测晚期非小细胞肺癌(NSCLC)患者外周血EGFR和KRAS基因突变的价值。方法采用COLD-PCR/Sanger对67例晚期NSCLC患者外周血中EGFR及KRAS基因突变进行检测,同时采用普通PCR/Sanger法进行平行检测。结果 67例样本中,COLD-PCR/Sanger检测EGFR的突变率高于普通PCR/Sanger法(43.3%比26.87%,P<0.05),主要表现在外显子19、21的突变,二者的一致性较好(Kappa=0.582);COLD-PCR/Sanger检测KRAS的突变率高于普通PCR/Sanger法(16.4%比5.97%,P<0.05),二者的一致性较好(Kappa=0.49)。结论 COLD-PCR/Sanger是一种高灵敏度检测肺癌患者外周血EGFR和KRAS基因突变的方法。Objective To evaluate the value of COLD-PCR/Sanger in detecting EGFR and KRAS mutations in peripheral blood of patient with non-small cell lung cancer（NSCLC）.Methods EGFR and KRAS mutations in peripheral blood samples of 67 patients with NSCLC were detected by COLD-PCR/Sange and common PCR/Sanger,respectively.Results The mutation rates of EGFR and KRAS detected by COLD-PCR/Sanger（43.3% and 16.4%,respectively）were higher than those detected by common PCR/Sanger（26.87% and 5.97%,respectively）.These two methods had a better consistency for the detection of EGFR and KRAS mutations（Kappa=0.582 and 0.49,respectively）.The mutations mainly occurred in exons 19 and 21.Conclusion COLD-PCR/Sanger is highly sensitive for detecting EGFR and KRAS mutations in patients with lung cancer.

关 键 词：非小细胞肺癌 COLD-PCR/Sanger EGFR基因突变 KRAS基因突变 

分 类 号：R734.2[医药卫生—肿瘤]