锗浓度对蛹虫草锗的积累和多糖化锗生物转化合成的影响  

Effects of Germanium Concentrations on Germanium Accumulation and Biotransformation of Polysaccarified Germanium in Cordyceps militaris

作　　者：王菊凤[1] 李鹄鸣[1] 杨道德[2] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]中南林业科技大学野生动植物保护研究所,湖南长沙410004

出　　处：《中药材》2015年第11期2331-2334,共4页Journal of Chinese Medicinal Materials

基　　金：国家科技部中小企业技术创新基金资助项目(11C26214302946);国家人事部资助项目(人事部文件国人部发[2004]99号);湖南省生态学省级重点学科项目(12JD016)

摘　　要：目的：研究不同锗浓度对蛹虫草锗的积累和多糖化锗生物转化合成的影响。方法：采用固体培养和液体培养方法。结果：对于子实体来说,当锗浓度为200 mg/L时,蛹虫草子实体的生物量最大;锗浓度为300 mg/L时,子实体多糖化锗的生物转化合成量最多;锗浓度为250 mg/L时,子实体有机锗转化率最高。对于菌丝体来说,当锗浓度为250 mg/L时,蛹虫草菌丝体的生物量最大;锗浓度为150 mg/L时,菌丝体多糖化锗的生物转化合成量最多;锗浓度为200 mg/L时,菌丝体有机锗转化率最高。结果显示锗浓度在150~300 mg/L时,比较适合蛹虫草锗的积累和多糖化锗的生物转化。蛹虫草子实体富锗能力比菌丝体强。结论：不同锗浓度能显著影响蛹虫草锗的积累和多糖化锗的生物转化（P〈0.05）。这一实验结果可为蛹虫草子实体的富锗栽培和开发利用提供参考。Objective： To study the effects of Germanium（ Ge） concentration on Ge accumulation and biotransformation of polysaccarified Ge（ PG） in Cordyceps militaris. Methods： Solid and liquid culture were used in this study. Results： In the solid culture conditions,when the Ge concentration of medium was 200 mg / L,the sporophore biomass of Cordyceps militaris was the maximum; and when Ge concentration was 300 mg / L,the amount of biotransformation of PG in sporophore was the highest; and when the Ge concentration is250 mg / L,conversion rate of organic germanium（ OG） in sporophore reached the highest value. In the liquid culture conditions,when the Ge concentration was 250 mg / L,the mycelium biomass of Cordyceps militaris was the maximum; and when Ge concentration was 150 mg / L,the amount of organic conversion of PG in mycelium was the most; and conversion rate of OG in mycelium was the highest in media with the Ge concentration of 200 mg / L. This study showed the germanium concentrations in 150 ~ 300 mg / L was more suitable for Ge accumulation and biotransformation of PG in Cordyceps militaris. In general,the biotransformation capacity to germanium of sporophore was stronger than that of mycelium of Cordyceps militaris. Conclusion： Germanium can significantly affect Ge accumulation and biotransformation of PG in Cordyceps militaris（ P〈 0. 05） at different concentration. This result has practical value for Ge enriched cultivation of fruiting body in Cordyceps militaris.

关 键 词：锗浓度 蛹虫草 锗富集 多糖化锗 

分 类 号：R284.1[医药卫生—中药学]