丹参素抑制RANKL诱导的破骨细胞分化  

Danshensu Suppresses RANKL-induced Osteoclastogenesis in Vitro

作　　者：沙鑫[1] 李锋[1] 吴太林 马文瑞[1] 李寰[2] 陶惠人[1] 

机构地区：[1]第四军医大学西京医院骨科,陕西西安710032 [2]第四军医大学西京医院心血管内科,陕西西安710032

出　　处：《现代生物医学进展》2015年第36期7017-7020,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81170256;81070698)

摘　　要：目的:研究丹参素对RANKL诱导的破骨细胞分化的影响。方法:运用冲洗法从股骨、胫骨中获得小鼠骨髓源性单核巨噬细胞用于体外RANKL诱导的破骨细胞分化,同时,施加不同剂量的丹参素干预,经TRAP染色法在形态学上观察观,蛋白印迹法检测蛋白水平的变化,实时定量PCR检测m RNA水平变化来研究丹参素对RANKL诱导的骨髓源单核巨噬细胞破骨分化的影响。结果:1不同剂量丹参素干预组与对照组相比,TRAP阳性破骨细胞数量得到了明显抑制(P<0.05)。2不同剂量丹参素干预组与对照组相比,磷酸化Akt的上调量被明显的降低。磷酸化p38 MAPK,JNK和ERK的变化则不明显。3不同剂量丹参素干预组与对照组相比,c-fos,TRAP,CTSK等参与破骨细胞分化的重要基因表达减少,NFATc1变化不明显。结论:丹参素通过下调磷酸化Akt水平的途径抑制了RANKL诱导的破骨细胞分化。Objective： To evaluate the effects of Danshensu（DSS） on the RANKL-induced osteoclastogenesis and the mechanism in vitro. Methods： Bone marrow cells were obtained by flushing the femurs and tibiae of mice, the non-adherent cells were collected to differentiate in the presence or absence of Danshensu and RANKL. Count the number of the TRAP＋ multinucleated osteoclasts, western bolt analysis and quantitative real-time PCR analysis. Results：1The number of osteoclast decreased comparing differernt dose danshensu group with control group. 2 The phosphorylation of Akt upregulated by RANKL-induced degraded by danshensu with different dose comparing to control group, while no significant difference were found in phosphorylation of ERK, p38 MAPK and JNK.3We found the expression of m RNA of the osteoclastic marker genes（TRAP, CTSK） were attenuated by addition of danshensu comparing to control group, as well as c-fos, but not seen in NFATc1. Conclusions： Danshensu suppress RANKL-induced osteoclastogenesis by downgrading phosphorylation of Akt.

关 键 词：丹参素 核激活因子κB受体 破骨细胞 细胞培养 

分 类 号：R-33[医药卫生] R285.5