河豚毒素荧光偏振免疫分析方法的建立  

Development of fluorescence polarization immunoassay for tetrodotoxin detection

作　　者：孙成彪 许娜[2] 刘洋[3] 肖世峰 黄燕霞[4] 韩奇[2] 李忠义[4] 万家余[4] 沈明浩[1] 刘文森[4] 

机构地区：[1]吉林农业大学食品科学与工程学学院,吉林长春130118 [2]吉林医药学院,吉林吉林市1320003 [3]吉林大学白求恩医学院药理学系,吉林长春130021 [4]军事医学科学院军事兽医研究所吉林省人兽共患病防控重点实验室,吉林长春130122

出　　处：《中国兽医学报》2016年第3期459-463,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372487)

摘　　要：河豚毒素主要存在于河豚鱼中,是一种致命的神经毒素。将量子点标记于河豚毒素,并将其作为示踪物,获得高且稳定的荧光信号,建立荧光偏振免疫分析技术检测河豚毒素。所建立的方法检测河豚毒素检测范围为1.36~101.27μg/L,IC50值为11.76μg/L,检测限为0.67μg/L。在该方法的检测范围内标准平均回收率为（99.91±2.60）%,变异系数为2.60%。与竞争性ELISA相比较,本方法河豚毒素测定值与竞争性ELISA测定值呈线性相关,且具有简单、快速等特点,可应用于临床诊断和食品安全检测。Tetrodotoxin is a fatal neurotoxin which exists in puffer fish.In this study,Quantum dots was linked to TTX as the tracer to achieve high and stable fluorescence signal and a fluorescence polarization immunoassay（FPIA）was developed for the detection of TTX.The developed FPIA for TTX had a detect range from 1.36-101.27μg/L,with an IC50 value of 11.76μg/L and the limit of detection（LOD）value of 0.67μg/L.The recovery test result was（99.91±2.60）%,with the CV value of 2.60%.The developed FPIA had a good correlation with competitive ELISA.The method was simple and fast,which could be applied in clinical diagnoses and food safety detection.

关 键 词：荧光偏振免疫分析 河豚毒素 量子点 

分 类 号：S859.8[农业科学—临床兽医学]