GST(phi) gene from Macrophyte Lemna minor is involved in cadmium exposure responses  

作　　者：陈世华 陈新 窦伟红 王亮 尹海波 郭善利 

机构地区：[1]Key Laboratory of Plant Molecular & Developmental Biology, College of Life Sciences, Yantai University

出　　处：《Chinese Journal of Oceanology and Limnology》2016年第2期342-353,共12页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.31370296);the Shandong Provincial Natural Science Foundation of China(Nos.Y2007D34,ZR2011CM044);the Key Technologies R&D Program of Shandong Province of China(No.2010GNC10937)

摘　　要：Reactive oxygen species （ROS） scavengers, including ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, are the most commonly used biomarkers in assessing an organisms＇ response to many biotic and abiotic stresses. In this study, we cloned an 866 bp GST（phi） gene in Lemna minor and investigated its characteristics, expression and enzymatic activities under 75 lamol/L cadmium concentrations in comparison with other ROS scavengers. GST（phi） gene expression patterns were similar to those of other scavengers of ROS. This suggests that GST（phi） might be involved in responding to heavy metal （cadmium） stress and that its expression level could be used as a bio-indicator in monitoring cadmium pollution.Reactive oxygen species(ROS) scavengers, including ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, are the most commonly used biomarkers in assessing an organisms' response to many biotic and abiotic stresses. In this study, we cloned an 866 bp GST( phi) gene in Lemna minor and investigated its characteristics, expression and enzymatic activities under 75 μmol/L cadmium concentrations in comparison with other ROS scavengers. GST( phi) gene expression patterns were similar to those of other scavengers of ROS. This suggests that GST( phi) might be involved in responding to heavy metal(cadmium) stress and that its expression level could be used as a bio-indicator in monitoring cadmium pollution.

关 键 词：Lemna minor GST（phi） gene heavy metal gene expression BIO-INDICATOR 

分 类 号：X173[环境科学与工程—环境科学]