Rapid Determination of Propofol Content in Rat Whole Blood with Capillary Microextraction and Fluorescence Spectrometry  

作　　者：Li LI Qingzhen WANG 

机构地区：[1]College of Pharmacy,Xinjiang Medical University

出　　处：《Agricultural Biotechnology》2016年第1期8-10,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(81260485)

摘　　要：[ Objective] This study aimed to establish a new method for determination of propofol content in rat whole blood with capillary microextraction and fluo- rescence spectrometry. [ Method ] The effects of extractant, extraction time, capillary length, capillary diameter and protein precipitant on extraction rate of propo- fol from rat whole blood were investigated. [ Result] The optimal extraction conditions were： 5 ml of cyclohexane as extractant, 1 ml of methanol as protein precipitant, extraction time 3 min. Under the optimal extraction conditions, the average extractive rate of propofol was higher than 99%. The linear range of the estab- lished method was 0.05 - 10.0ug/ml, and the relative standard deviation was less than 2%. [ Conclusion~ Extracting propofol from blood samples using capillary microreactor is convenient and feasible.[ Objective] This study aimed to establish a new method for determination of propofol content in rat whole blood with capillary microextraction and fluo- rescence spectrometry. [ Method ] The effects of extractant, extraction time, capillary length, capillary diameter and protein precipitant on extraction rate of propo- fol from rat whole blood were investigated. [ Result] The optimal extraction conditions were： 5 ml of cyclohexane as extractant, 1 ml of methanol as protein precipitant, extraction time 3 min. Under the optimal extraction conditions, the average extractive rate of propofol was higher than 99%. The linear range of the estab- lished method was 0.05 - 10.0ug/ml, and the relative standard deviation was less than 2%. [ Conclusion~ Extracting propofol from blood samples using capillary microreactor is convenient and feasible.

关 键 词：PROPOFOL Whole blood Capillary microreactor EXTRACTION 

分 类 号：R614[医药卫生—麻醉学]