恒波长同步荧光猝灭法测定痕量亚硝酸根  

Determination of trace nitrite by constant-wavelength synchronous fluorescent quenching method

作　　者：李岚[1] 刘金燕[1] 

机构地区：[1]嘉应学院化学与环境学院,梅州514015

出　　处：《中国食品添加剂》2016年第2期146-149,共4页China Food Additives

摘　　要：在HCl介质中,Δλ=20nm,亚硝酸根对溴酸根氧化罗丹明B具有催化作用,使罗丹明B同步荧光发生猝灭,且猝灭程度与亚硝酸根浓度能形成线性关系,据此建立了恒波长同步荧光猝灭测定微量亚硝酸根的新方法。方法的检出限为1.3ng/m L,线性范围为2μg/L^52μg/L。该方法可用于火腿肠中亚硝酸根含量的测定能得到满意结果。In HCl（Δλ=20nm）,constant-wavelength synchronous fluorescence of Rhodamine B can be quenched using nitrite as catalyst for the oxidation of Rhodamine B with bromate ion,and the extent of quenching and the content of nitrite showed a liner relationship. A new method for the determination of trace nitrite was developed based on the above observation. The detective limit for nitrite was 1.3ng/mL with linear range of 2μg/L^52μg/L. The method used in determining trace NO2-in sausages had a satisfactory results.

关 键 词：恒波长同步荧光猝灭法 罗丹明B 亚硝酸根 溴酸根离子 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS207.5[轻工技术与工程—食品科学与工程]