前列腺癌组织中TRIB1基因和蛋白异常表达与患者不良预后的相关性  

Increased expression of tribbles pseudokinase 1(TRIB1) mRNA and protein predicts malignant progression and adverse clinical outcomes in patients with prostate cancer

作　　者：万跃平[1] 习明[1] 万颂 华伟[1] 曾昭昌 刘远灵[1] 林卓远 吴永定 杨盛帮[2] 韩兆冬[2] 

机构地区：[1]南方医科大学附属花都医院泌尿外科,广州510800 [2]广州医科大学附属广州市第一人民医院泌尿外科和广东省临床分子医学及分子诊断重点实验室,广州510180

出　　处：《岭南现代临床外科》2016年第1期87-91,共5页Lingnan Modern Clinics in Surgery

基　　金：广东省自然科学基金项目(2014A030310066);广州市医药卫生科技项目(20141A010013)

摘　　要：目的研究Tribbles同源蛋白1(TRIB1)在良性前列腺增生和前列腺癌中的表达,并分析其表达水平与患者临床特征及预后的关系。方法在公共基因芯片数据库(GEO)中下载前列腺样本中的相关基因芯片数据,分析TRIB1在前列腺样本中mRNA的表达水平。收集临床手术切除或者穿刺活检的前列腺癌和前列腺增生组织,通过免疫组化检测前列腺癌、前列腺增生组织中TRIB1蛋白的表达。分析TRIB1蛋白和基因的表达水平与患者临床特征及预后的关系。结果前列腺癌患者组织中TRIB1蛋白表达显著上调(P<0.01),TRIB1蛋白表达上调与前列腺癌Gleason评分(P<0.01)、病理分期(P=0.02)相关,基因芯片数据提示TRIB1基因表达上调与前列腺癌Gleason评分(P=0.02)、病理分期(P=0.01)、无生化复发生存率(BCR-free survival)(P=0.047)相关。结论 TRIB1在前列腺癌组织中表达上调,前列腺组织中检测TRIB1可能有助于判断前列腺癌分化程度并评估预后。Objective To study the expression and significances of TRIB1 in benign prostate hyperplasia（BPH） tissues and prostate cancer tissues, and to illustrate the correlation with clinical significance and outcomes. Methods The dataset of whole genomic expression profiles got from the prostate tissues were obtained from gene expression omnibus（GEO） database. The samples were derived from benign prostatic tissues and prostatic carcinoma tissues of the patients with BPH or prostatic cancer..Immunohistochemistry analyses were conducted to detect the expression of TRIB1 protein in both prostate cancer and BPH tissues,.and further statistically analyzed the correlations with clinic pathological characteristics and outcomes of the patients with prostate cancer. Results The expression of TRIB1 protein was significantly increased in prostate cancer tissues compared with BPH tissues（P 〈0.01）..The expression rate of TRIB1 protein was in relation with Gleason score（P 〈0.01）and clinical stage（P =0.02）. The upregulation of CENPF m RNA expression in the PCa tissues significantly correlated with a higher Gleason score.（P =0.02）,.an advanced pathological stage.（P =0.01）.and poor biochemical recurrence.（BCR）-free survival.（P =0.047）. Conclusion Our study showed the increased expression of both TRIB1 protein and m RNA levels plays an important role in the progression of PCa. More importantly, the increased expression of TRIB1 may efficiently predict poor BCR-free survival in patients with PCa.

关 键 词：TRIB1 前列腺癌 GLEASON评分 无生化复发生存 组织芯片 

分 类 号：R737.25[医药卫生—肿瘤]