The Survey of Cronobacter spp.(formerly Enterbacter sakazakii) in Infant and Follow-up Powdered Formula in China in 2012  

作　　者：PEI Xiao Yan YAN Lin ZHU Jiang Hui LI Ning GUO Yun Chang FU Ping JIA Hua Yun ZHANG Xiu Li YANG Xiao Rong YANG Da Jin 

机构地区：[1]China National Center for Food Safety Risk Assessment, Beijing 100022, China [2]Hunan Province Center for Disease Control and Prevention, Changsha 410000, Hunan, China [3]Henan Province Center for Disease Control and Prevention, Zhengzhou 450000, Henan, China [4]Sichuan Province Center for Disease Control and Prevention, Chengdu 610000, Sichuan, China

出　　处：《Biomedical and Environmental Sciences》2016年第2期99-106,共8页生物医学与环境科学（英文版）

基　　金：supported by National High-tech R&D Program of China(863 Program)(2012AA101603)

摘　　要：Objective To determine Cronobacter spp. contamination in infant and follow-up powdered formula in China. Methods All of 2282 samples were collected from the retail markets in China from January 2012 to December 2012, and analyzed for Cronobacter spp. by the Chinese National Food Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis（PFGE） with XbaI and Spe I restriction enzymes. Results Cronobacter spp. strains were isolated from 25 samples, and the positive rates in infant powdered formulas and follow-up powdered formulas were 0.90%（10/1011） and 1.18%（15/1271）, respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization of Cronobacter spp. to elucidate genetic diversity revealed only three pairs of Cronobacter spp. out of 25 having the same PFGE patterns. Conclusion The current investigation indicated a lower positive rate of Cronobacter spp. in the powdered formula in China. This evidence suggested contamination originating from multiple different sources during the manufacturing process.Objective To determine Cronobacter spp. contamination in infant and follow-up powdered formula in China. Methods All of 2282 samples were collected from the retail markets in China from January 2012 to December 2012, and analyzed for Cronobacter spp. by the Chinese National Food Safety Standard. Characterization of the isolates was analyzed by pulsed-field gel electrophoresis（PFGE） with XbaI and Spe I restriction enzymes. Results Cronobacter spp. strains were isolated from 25 samples, and the positive rates in infant powdered formulas and follow-up powdered formulas were 0.90%（10/1011） and 1.18%（15/1271）, respectively. Analysis of variable data regarding different purchasing store formats, seasonality, and production locations as well as comparison of infant versus follow-up formulas did not reveal statistically significant factors. During the sampling period, one of six surveillance zones did exhibit a statistically significant trend towards higher positive rate. PFGE characterization of Cronobacter spp. to elucidate genetic diversity revealed only three pairs of Cronobacter spp. out of 25 having the same PFGE patterns. Conclusion The current investigation indicated a lower positive rate of Cronobacter spp. in the powdered formula in China. This evidence suggested contamination originating from multiple different sources during the manufacturing process.

关 键 词：Cronobacter spp Contamination PFGE Powdered formula 

分 类 号：R155.5[医药卫生—营养与食品卫生学]