淫羊藿苷对腺样囊性癌细胞Bcl-2、Bax表达的影响  

Effects of icariin on the expression of Bcl-2、Bax in salivary adenoid cystic carcinom

作　　者：王雨[1] 李萍[2] 李亦婕[3] 刘昕[2] 王越[2] 

机构地区：[1]江汉大学护理与医学技术学院,湖北武汉430056 [2]遵义医学院附属口腔医院,贵州遵义563000 [3]西安医学院口腔系,陕西西安710021

出　　处：《临床口腔医学杂志》2016年第2期75-79,共5页Journal of Clinical Stomatology

摘　　要：目的:1探讨淫羊藿苷体外抑制涎腺腺样囊性癌细胞(SACC-M)的最佳浓度和时间点及作用于SACC-M后相关细胞因子Bcl-2、Bax的表达变化。方法:1以不同浓度(0μg/m L、6.25μg/m L、12.5μg/m L、25μg/m L、50μg/m L、100μg/m L、200μg/m L、400μg/m L、800μg/m L)的淫羊藿苷分别作用于SACC-M(24 h、48 h、72 h),选择淫羊藿苷对高转移SACC-M细胞株的最佳作用浓度和作用时间,通过形态学观察淫羊藿苷对SACC-M细胞形态的改变。2正常对照组中加入同种剂量的药物溶剂,实验组中加入不同浓度的淫羊藿苷处理高转移SACC-M细胞株,免疫组化检测Bcl-2、Bax表达量的变化。结果:1与对照组(0μg/m L)相比较,淫羊藿苷在不同浓度(50、100、200、400、800μg/m L)对SACC-M细胞株的增殖具有抑制作用(P<0.05)。在同一药物浓度下,从50μg/m L浓度开始,与同浓度24 h淫羊藿苷比较有统计学意义(P<0.05),但到了800μg/m L浓度时已经没有时间依赖性(P>0.05)。当用(50、100、200、400μg/m L)作用于细胞24 h后,显微镜下观察显示ACC-M细胞具有典型的凋亡细胞形态学特征。2淫羊藿苷能呈浓度依赖性下调ACC-M中Bcl-2同时上调Bax蛋白的表达。结论:体外淫羊藿苷可以抑制ACC-M的增殖,其机制可能与下调Bcl-2和上调Bax的表达有关。Objective：In vitro cultivation of SACC-M cells and explore the best concentration of icariin and best point in time on anti SACC-M as well as study the alteration of Bcl-2、Bax protein after SACC-M cells were treated by icariin. Method：1The SACC-M cells after exposure to different concentrations of icariin（0 μg/m L、6.25 μg/m L、12.5μg/m L、25 μg/m L、50 μg/m L、100 μg/m L、200 μg/m L、400 μg/m L、800 μg/m L）for different time periods（24 h、48 h、72 h） was determined by MTT assay,then explore the best concentration of icariin and best point in time on anti SACC-M cells. And through the morphological observation test ICA’s effect on salivary gland adenoid cystic cancer cells apoptosis;2Compare the protein expression discrepancy of Bax、Bcl-2 in non- icariin-treat and icariin- treat SACC-M cells which after incubation with best concentrations of icariin and best point in time were detected by using Immunohistochemistry. Result：1Compare with the control group（0 μg/m L）,icariin in different concentrations（50 μg/m L、100 μg/m L、200 μg/m L、400 μg/m L、800 μg/m L）icariin can inhibit proliferation of cancer cells（P 〈0.05）,compared with others of 24 h action which were in the same concentration,it was statistically significant from 50μg/ml concentration（P 〈0.05）,but was of no time dependence till it’s concentration to the 800 μg/m L concentration（P 〉0.05）.When use（50 μg/m L、100 μg/m L、200 μg/m L、400 μg/m L） icariin in cells after 24 h,the ACC-M cells presented typical morphological features of apoptosis by microscope.2Icariin depend on concentration of inducing salivary gland adenoid cystic cancer cells apoptosis,the higher the concentration,the higher apoptosis rate,lower expression of Bcl-2 and rise the expression of Bax.Conclusion：Epimedium glycoside can inhibit the growth of ACC-M in vitro,its mechanism may be related to lower expression of Bcl-2 and rise the expression of Bax.

关 键 词：涎腺腺样囊性癌 淫羊藿苷 BCL-2 BAX 

分 类 号：Q786[生物学—分子生物学] R739.87[医药卫生—肿瘤]